前列腺素E_1在大鼠肺缺血再灌注损伤中的作用研究

目的:探讨前列腺素E1在肺缺血再灌注损伤(IRI)中的作用。方法:取SD大鼠60只,随机均分为正常对照组、假手术组、缺血再灌注组(I/R组)和前列腺素E1(PGE1)高、中、低剂量组(20、10、5μg·kg-1);假手术组和I/R组给予0.5mL生理盐水尾静脉注射,其余各组给予相应药物;给药1h后建立肺IRI模型,再灌注6h后处死大鼠提取标本。检测各组肺组织湿/干重比(W/D)、丙二醛(MDA)、髓过氧化物酶(MPO)、核因子-κB(NF-κB)水平。结果:与正常对照组和假手术组比较,I/R组W/D、MDA、MPO和NF-κB水平显著升高(P<0.05);与I/R组比较,PGE1中、高剂量组肺W/D、MDA、MPO和NF-κB水平显著降低(P<0.05或P<0.01)。结论:PGE1可能通过抑制氧化应激和调节炎症反应对肺IRI产生保护作用。

Effects of Prostaglandin E1 on Lung Ischemia-reperfusion Injury in Rats

OBJECTIVE： To investigate the effects of prostaglandin E1 （PGE1） on lung ischemia-reperfusion injury （IRI） in rats. METHODS： 60 SD rats were divided into normal control group, sham operation group, UR group and PGE1 high dosage group （HD）, medium dosage group （MD） and low dosage group （LD） randomly. Sham operation group and UR group were given normal saline 0.5 mL via tail vein injection and other groups were given relevant medicine. 1 h after administration, lung IRI model was established. Rats were sacrificed 6 h after the reperfusion. The wet and dry weight （W/D） of lung tissue, the content of malondialdehyde （MDA）, the activity of myeloperoxidase （MPO） and the content of NF-κB were detected by spectrophotometer and ELISA method. RESULTS ： Compared with normal control group and sham operation group, the level of W/D, MDA, MPO and NF-KB in I/R group increased significantly （P〈0.05）. Compared with I/R group, the levels of W/D, MDA, MPO and NF-κB in PGE1 MD group and PGE1 HD group decreased significantly （P〈0.05 or P〈0.01）. CONCLUSION： PGE1 has a protective effect on lung IRI, which may be related to the inhibition of oxidative stress and regulating the inflammation response.