| 紫外分光光度法测定大蝎子草药材中总黄酮的含量 |
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| 引用本文： | 刘文炜,沈祥春,陶玲,高玉琼. 紫外分光光度法测定大蝎子草药材中总黄酮的含量[J]. 中国药房, 2010, 0(43): 4088-4089 |
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| 作者姓名： | 刘文炜 沈祥春 陶玲 高玉琼 |
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| 作者单位： | [1]贵阳医学院,贵阳市550004 [2]贵州省生物技术研究开发基地,贵阳市550002 |
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| 基金项目： | 贵州省科学技术基金项目（黔科合J字[2008]2162号）; 贵州省优秀科技教育人才省长专项资金项目（黔省专合字（2006）55号） |
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| 摘 要： | 目的:建立大蝎子草总黄酮含量测定方法。方法:采用紫外分光光度法,以芹菜素为标准品,检测波长为268nm。结果:芹菜素检测浓度在1.984～11.904μg·mL-1范围内与吸光度呈良好的线性关系(r=0.9999);平均加样回收率为96.15%,RSD=1.98%(n=6)。结论:本方法稳定、准确、可靠,可用于大蝎子草总黄酮的含量测定。
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| 关 键 词： | 大蝎子草 芹菜素 总黄酮 紫外分光光度法 含量测定 |
Determination of Total Flavonoids from Girardinia diversifolia by UV Spectrophotometry |
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| LIU Wen-wei,SHEN Xiang-chun,TAO Ling,GAO Yu-qiong. Determination of Total Flavonoids from Girardinia diversifolia by UV Spectrophotometry[J]. China Pharmacy, 2010, 0(43): 4088-4089 |
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| Authors: | LIU Wen-wei SHEN Xiang-chun TAO Ling GAO Yu-qiong |
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| Affiliation: | (Guiyang Medical University,Guiyang 550004,China)(Guizhou Institute of Biotechnology Research and Development,Guiyang 550002,China) |
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| Abstract: | OBJECTIVE：To establish the determination method for total flavonoids from Girardinia diversifolia.METHODS：The contents of total flavonoids were determined by UV spectrophotometry at detection wavelength of 268 nm with apigenin as standard substance.RESULTS：The linear range of apigenin were 1.984～11.904μg·mL -1（r=0.999 9）with average recovery of 96.15%（RSD=1.98%,n=6）.CONCLUSION：The method is accurate,reliable and stable and it can be used for the content determination of total flavonoids from G.diversifolia. |
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| Keywords: | Girardinia diversifolia Apigenin Total flavonoids UV spectrophotometry Content determination |
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