| 葛根种质资源的分子地理标识研究 |
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| 引用本文： | 刘东吉,余智奎,刘春生,王学勇. 葛根种质资源的分子地理标识研究[J]. 中国中药杂志, 2011, 36(3): 299-301 |
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| 作者姓名： | 刘东吉 余智奎 刘春生 王学勇 |
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| 作者单位： | 北京中医药大学中药学院,北京,100102 |
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| 基金项目： | 国家自然科学基金项目(30672615) |
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| 摘 要： | 目的:利用葛根的ITs,psbK-psbI,trnH-psbA序列信息对不同产地葛根进行分类,为分析不同产地的葛根药材提供依据.方法:采集东北、华北、华中和西北共8个居群24份葛根样品,提取DNA,扩增ITS序列、psbK-psbI及trnH-psbA序列、测序,通过序列比对,根据差异划分基因型或单倍型.结果:ITS1,ITS2序列各有1个单碱基突变,5.8S序列有1个单碱基突变;psbK-psbI序列有2个单碱基突变;trnH-psbA序列有1个单碱基突变,还有1段10个碱基的缺失突变.结论:利用ITS序列将葛根划分成4个基因型,根据psbK-psbI和trnH-psbA序列均将葛根划分成2个单倍型,为进一步评价提供依据.
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| 关 键 词： | 葛根 不同产地 分类 |
| 收稿时间： | 2010-10-13 |
Classification of Pueraria lobata in different geographical regions |
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| LIU Dongji,YU Zhikui,LIU Chunsheng and WANG Xueyong. Classification of Pueraria lobata in different geographical regions[J]. China Journal of Chinese Materia Medica, 2011, 36(3): 299-301 |
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| Authors: | LIU Dongji YU Zhikui LIU Chunsheng WANG Xueyong |
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| Affiliation: | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China |
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| Abstract: | Objective : To classify Pueraria lobata originated from different geographical regions based on ITS,psbK-psbI and trnH-psbA information. Method : Twenty-four samples of P. lobata were collected from northeast China, north China, central China and northwest China. DNA extraction, PCR,sequence and genotypes/ haplotypes analysis were performed . Result : ITS1, 5.8S and ITS2 varied only 1 bp respectively, psbK-psbI 2 bps; trnH-psbA varied 1 bp and 10 bp deletion. Conclusion : Based on the variation of ITS,psbK-psbI and trnH-psbA, 4 genotypes and 2 haplotypes were identified, respectively. |
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| Keywords: | Pueraria lobata DNA sequence classification |
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