| 乙型肝炎病毒表面抗原终止密码子的偏嗜性选择 |
| |
| 引用本文： | 李俊强,田敬华,刘峰,杜绍财. 乙型肝炎病毒表面抗原终止密码子的偏嗜性选择[J]. 北京大学学报(医学版), 2008, 40(3): 270-272 |
| |
| 作者姓名： | 李俊强 田敬华 刘峰 杜绍财 |
| |
| 作者单位： | (1.北京大学人民医院,北京大学肝病研究所,北京 100044;2.北京地坛医院) |
| |
| 摘 要： | 目的:探究乙型肝炎病毒表面抗原(HBsAg)终止密码子的存在状况及偏嗜性.方法:从GenBank中选取各基因型的参考序列共174例;使用生物软件BioEdit进行序列比对,应用RNAdraw构建并分析其RNA二级结构.结果:(1)在174例参考序列中,HBsAg的终止密码子共有TAA和TGA两种形式.其中TAA形式的有124例,占71.26%;TGA形式的有50例,占28.74%.(2)终止密码子的使用存在偏嗜性,并影响RNA的二级结构和蛋白质的编码氨基酸序列.结论:HBsAg终止密码子的使用偏嗜性客观存在,可能与进化过程中RNA结构和蛋白质功能的保守性相关.
|
| 关 键 词： | 肝炎病毒 乙型 密码子 终止 序列分析 |
| 文章编号： | 1671-167X(2008)03-0270-03 |
| 修稿时间： | 2007-08-27 |
Hepatitis B surface antigen terminates codon bias selection |
| |
| LI Jun-qiang,TIAN Jing-hua,LIU Feng,DU Shao-cai. Hepatitis B surface antigen terminates codon bias selection[J]. Journal of Peking University. Health sciences, 2008, 40(3): 270-272 |
| |
| Authors: | LI Jun-qiang TIAN Jing-hua LIU Feng DU Shao-cai |
| |
| Affiliation: | Hepatology Institute, Peking University People's Hospital, Beijing, China. |
| |
| Abstract: | OBJECTIVE: To investigate the existence condition of hepatitis B surface antigen(HBsAg) termination codon bias. METHODS: A total of 174 reference sequences of all kinds of Hepatitis B virus(HBV) genotypes were chosen from GenBank, and compared by BioEdit. Then secondary structure of RNA was constructed and analyzed together. RESULTS: (1) There were two types of HBsAg termination codon: TAA and TGA in 174 reference sequences. TAA was in 124 cases (71.26%); and TGA in 50 cases (28.74%). (2) There was codon bias selection in HBsAg termination codon, and it could affect the secondary structure of RNA and amino acid sequence encoding protein. CONCLUSION: HBsAg termination codon bias exists and may be related to RNA structure and the conservation of protein function in the evolutionary progress. |
| |
| Keywords: | Hepatitis B virus Codon termination Sequence analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《北京大学学报(医学版)》浏览原始摘要信息 |
|
点击此处可从《北京大学学报(医学版)》下载全文 |
