蓝莓对大鼠免疫性肝纤维化超微结构及CYP2E1表达的影响

目的:观察蓝莓对大鼠免疫性肝纤维化超微结构及细胞色素P4502E1(CYP2E1)表达的影响。方法:50只雄性Wistar大鼠随机分为正常组、模型组、蓝莓原浆预防组(蓝莓组)、复方鳖甲软肝片预防组(复方组)、蓝莓原浆加复方鳖甲软肝片预防组(蓝莓+复方组)。猪血清复制大鼠肝纤维化模型,各预防组在造模同时给予蓝莓原浆或(和)复方鳖甲软肝片灌胃。12周后处死大鼠,观察肝组织超微结构变化,RT-PCR、Western Blot和免疫组织化学法检测大鼠肝组织CYP2E1 mRNA及蛋白质的表达。结果:与模型组比,3个用药组肝纤维化程度明显减轻(P0.05),超微结构病变明显改善,肝组织CYP2E1 mRNA及蛋白质表达无明显差异。结论:蓝莓对大鼠免疫性肝纤维化有一定的预防作用,对肝细胞超微结构病变有一定的保护作用,对CYP2E1的表达无明显影响。

Effects of blueberry on ultrastructural and expression of cytochrome CYP2E1 in rats with immune hepatic fibrosis

Objective: To study the effects of blueberry on ultrastructural and expression of cytochrome P450 2E1(CYP2E1) in rats with immune hepatic fibrosis.Methods:Fifty male Wistar rats were randomly divided into normal group,model group,blueberry juice prevention group(blueberry group),Fufang Biejia Ruangan Tablet prevention group(Compounel Group),blueberry juice + Fufang Biejia Ruangan Tablet prevention group(blueberry+Compounel Group).The hepatic fibrosis model of rat was established by porcine serum.Simultaneously,rats in prevention groups were respectively given orally blueberry juice or FFBJRG tablet or combinations of blueberry juice and FFBJRG tablet.All rats were killed at the end of the twelfth week.Ultrastructural changes in hepatocytes was evaluated,expression of hepatic CYP2E1 was detected by real-time reversed transcribed-polymerase chain reaction(RT-PCR),Western blot and immunohistochemical techniques.Results: Compared with model group,the stage of hepatic fibrosis in blueberry group was significantly weakened(P<0.05),ultrastructural alterations were markedly attenuated,the expression of CYP2E1 was not significantly different.Conclusion: Blueberry has protective effects on rat immune hepatic fibrosis and alleviates hepatocyte ultrastructural alterations,while it has no effect on the expression of CYP2E1.