脂联素对高糖中hRPE细胞活性及T—cad表达的影响

目的观察脂联素对高糖环境下人视网膜色素上皮（hRPE）细胞活性及T-钙黏蛋白（T—cad）表达的影响，以阐明脂联素和T—cad对糖尿病视网膜病变（DR）的影响。方法①将hRPE细胞随机分为正常对照组（5．5mmo~L葡萄糖）、高糖组（33mmol／L葡萄糖）、脂联素组（33mmol/L＋2．5μg／m1脂联素），运用MTT法测定不同情况下48h后对细胞活性的影响；②再将上述各组细胞分别培养24h、48h、72h后，运用荧光定量PCR测定T—cad的表达水平。结果①高糖组细胞活性降低，加入脂联素后细胞活力比高糖组升高；②随时间的延长，高糖组T—cad表达下降，脂联素组T—cad表达增加。结论脂联素可通过Tcad保护hRPE细胞的功能。

Effects of Adiponectin on Cell Activity and Expression of T - cadherin in Human RPE Cells Cultured with High D - glucose

Objective To investigate the effects of adiponectin on cell activity and expression of T - cadherin in human retinal pigment epithelial（hRPE） cells cultured with high glucose. Methods ①The hRPE cells were divided into three groups： normal glucose group（5.5mmol/L glucose） , high glucose group （33mmol/L glucose） , high glucose plus adiponectin group （ 33mmol/L glucose ＋ 2.5ug/ ml adiponectin）. 48h later, the cell activity was observed by MTT assay. ②After the above groups were cultured respectively for 24h ,48h, 72h again, the expression of T - cadherin mRNA was observed by RT - PCR. Results ①Cell activity in high glucose group significantly decreased. Cell activity adiponectin group was higher than that in the high glucose group.②With the extension of time , the expression of T- cadherin decreased in high glucose group, but increased in adiponectin group. Conclusion Adiponectin may be protect the hRPE cells by T - cadherin.