白芍总甙对应激大鼠下丘脑一垂体一肾上腺轴和免疫功能的调节

本文以血浆皮质酮（ＣＳ）、促肾上腺皮质激素（ＡＣＴＨ）和β－内啡肽（β－ＥＮＤ）含量为指标，观察了白芍总甙（ＴＧＰ）对不同状态（正常或不同应激）的大鼠下丘脑－垂体－肾上腺轴（ＨＰＡＡ）及其免疫功能的调节作用。结果表明，ＴＧＰ对正常大鼠的ＨＰＡＡ呈现小剂量（１２．５～５０．０ｍｇ·ｋｇ－１）兴奋（血浆ＣＳ含量升高）和大剂量（１００～２００ｍｇ·ｋｇ－１）抑制（血浆ＣＳ含量降低）的剂量依赖性调节作用。其次，ＴＧＰ可兴奋轻度应激（２０℃水游泳）大鼠的ＨＰＡＡ和抑制重度应激（４℃水游泳或２４ｈ束缚）大鼠的ＨＰＡＡ，使过高的血浆ＣＳ、ＡＣＴＨ和β－ＥＮＤ含量降低，提示ＴＧＰ对应激大鼠ＨＰＡＡ呈现轴机能依赖性的调节作用。此外，ＴＧＰ在降低束缚应激大鼠血浆ＣＳ、ＡＣＴＨ和β－ＥＮＤ水平的同时，上调受抑大鼠脾淋巴细胞ＣｏｎＡ增殖反应和腹腔Ｍ释放Ｈ２Ｏ２，提示ＴＧＰ的免疫调节作用可能与其调节ＨＰＡＡ的功能有关。

The modulatory effects of TGP on hypothalamo-pituitaryadrenal axis and immune function in stress rats

Total glucosides of paeony TGP either raised the excitability of HPAA (CS level raised) with small doses (12. 5￣ 50 mg·kg-1)or depressed the one of HPAA (CS level reduced) with large doses(100￣200mg·kg-1), indicating that it played an important role in the dose-dependent modulatory effects in normal rats'HPAA. Under lighter stress (swimming, 20℃, TGP excited the stimulated rat's HPAA. However, under heavier stress (swimming,4℃)it inhibited the over-stim-ulated rat's HPAA, which served as the protection of HPAA from exhaustion.These results show that TGP regulates the HPAA function, depending on the different activation state of this axis in stress rats. In addition TGP upregulated the deficient mitogenisis of T lymphocytes and the dinimished release of hydrogen peroxide from the peritoneal MΦs in restraint-stress rats,as well as down-regulated their elevated CS,ACTH and β-END levels in plasma, indicating that the effect of TGP on immune function relates to its modulatory effect on HPAA.