阿片κ受体在新生兔窒息中的作用

目的　探讨阿片κ受体在围生期缺氧导致新生兔窒息中的作用。方法　将 16只孕 30d母兔窒息后立即剖宫取出仔兔 ,随机分 3组 :新生兔窒息未治疗组 (窒息组 )、新生兔窒息中枢MR2 2 6 6治疗组 (MR1组 )及新生兔窒息外周MR2 2 6 6治疗组 (MR2组 ) ;MR1组及MR2组仔兔在分娩后 ,即刻分别从小脑延髓池及静脉注入阿片受体拮抗剂MR2 2 6 6。另取 4只无窒息母兔剖宫取出正常新生兔作为对照组。新生兔均于剖宫产后 5、10、15、30min进行呼吸、心跳、肤色、肌张力、反射评分。结果　MR1组及MR2组仔兔Apgar评分显著高于窒息组 (P<0 .0 5及P <0 .0 1) ,且MR2组评分明显高于MR1组 (P <0 .0 5 )。结论　阿片κ受体与围生期缺氧导致的新生兔窒息的发生发展密切相关

The role of opioid kappa-receptor in neonatal rabbit asphyxia

Objective To evaluate the role of opioid receptors in neonatal rabbit asphyxia.Methods Pregnant near-term rabbits were asphyxiated.The rabbit fetuses were delivered by cesarean section and all pups were asphyxiated ( all fetuses had scores of 2 to 7).The pups are divided randomiy into 3 groups: neonatal rabbit asphyxia untreated(asphyxia group),neonatal rabbit asphyxia treated with MR2266 (MR1 group) and neonatal rabbit asphyxia treated with cantre MR2266(MR2 group).The latter two groups were given an medulla pool and intravenous dose of the opiate antagonist MR2266 respectively.All the rabbits were scored according to respiration,heart beat,skin color,muscle tone,reflex at the 5t,10t,15t 30t minute after cesarean delivery.Results the MR-treated pups had significantly better Apgar scores than asphyxia untreated group(P<0.05 and P<0.01); there was statistical difference between MR1 group and MR2 group (P<0.05).Conclusions These results suggested that kappa-receptor participated in conducting the endogenous opioid peptides effect,it was associated with the pathophysiological changes in neonatal rabbit asphyxia.