非小细胞肺癌中DPC4基因的表达与微血管形成的关系

目的：探讨DPC4（deleted in pancreatic carcinoma locus4，DPC4）基因在非小细胞肺癌（NSCLC）中的表达及其与微血管形成的关系。方法：利用免疫组织化学S-P法检测52例NSCLC组织、19例相应的癌旁正常肺组织中DPC4、VEGF的表达，用CD34标记血管内皮细胞并计算微血管密度MVD值。结果：DPC4在肺癌原发灶中的阳性表达率为63．5％（33／52），与癌旁正常肺组织中的阳性表达率89．5％（17／19）相比，DPC4阳性表达水平显著降低（P〈0．05）；DPC4与组织学类型、肿瘤细胞分化程度无关（P〉0．05），但与淋巴结转移显著相关（P〈0．05）。52例NSCLC中，DPC4的表达与VEGF、MVD值均呈负相关（r=-0．303，P=0．020）。结论：DPC4的低表达可能是肺癌发生过程的早期事件，可促进肺癌的淋巴结转移，并可通过直接或间接的作用促进肺癌血管生成。

Expression of DPC 4 gene in non-small cell lung carcinoma and its relation to angiogenesis

Objective:To study the expression of DPC 4(deleted in pancreatic carcinoma locus 4,DPC 4)in non-small cell lung carcinoma as well as its association with angiogenesis.Methods:The expression of DPC 4,VEGF and CD34 was detected in 52 cases with primary NSCLC and 19 adjacent normal lung tissues by using immunohistochemical S-P method.Results:Positive expression rate of DPC 4 in primary lung cancer tissues was 63.5%(33/52),compared with the positive rate 89.5%(17/19)in adjacent normal lung tissues,the DPC 4 expression level was significantly degraded (P<0.05).The positive expression of DPC 4 had no correlation with tissue types and cellular differentiation (P>0.05),but it was closely associated with lymph node metastasis (P<0.05).In all 52 patients with NSCLC,the relationships between DPC 4 and VEGF or MVD,showed apparently negative correlation (r=-0.303,P=0.020).Conclusion:The low expression of DPC 4 could be an early event during the course of the NSCLC's development.Simultaneously,it also promoted lymph node metastasis by promoting the angiogenesis in NSCLC derectly or indirectly.