分泌IL-5T细胞杂交瘤的培养及IL-5的分离纯化 |
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引用本文: | 宋春华 米从明. 分泌IL-5T细胞杂交瘤的培养及IL-5的分离纯化[J]. 皖南医学院学报, 1995, 14(3): 189-190 |
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作者姓名: | 宋春华 米从明 |
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摘 要: | 高浓度IL-5可以从T细胞杂交瘤培养的上清液中获得。本文介绍分泌IL-5的T细胞杂交瘤的培养及其上清液中分高纯化IL-5的方法,提纯了高浓度的IL-5。
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关 键 词: | 杂交瘤;T-淋巴细胞;白细胞介素5 |
CULTURE OF IL-5-SECRETING T CELL HYBRIDOMA AND SEPARATION AND PURIFICATION OF IL-5 |
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Abstract: | High concentration of IL-5 can be purified from the supernatant of T cell hybridoma.In this article, the methods of culture of the T cell hybridoma and separation and purification of IL-5 were reperted. The resoults showed that it is an effective way to purify IL-5 with th NC-17 coated cloumn. |
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Keywords: | Hybridoma T-lymphocyte Interleukin-5 |
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