凋亡抑制基因 Survivin在肝癌细胞中的表达及其与PTEN、cyclin D1蛋白表达的关系

目的:探讨Survivin基因的表达在肝癌发生、发展中的作用及其与PTEN、cyclin D1蛋白表达的相互关系.方法:采用流式细胞术对肝细胞癌(48例)、癌旁肝硬化(32例)、肝硬化(6例)、正常肝组织(6例)Survivin、PTEN、cyclin D1基因蛋白的表达进行定量检测.结果:Survivin和cyclin D1蛋白表达的FI值明显高于癌旁组织、肝硬化和正常肝组织,而肝细胞癌的PTEN蛋白的FI值明显低于癌旁组织、肝硬化和正常肝组织.Survivin和PTEN蛋白的表达量与分化程度、预后有关,与肿瘤大小、门静脉癌栓、肝内转移无关.随着肿瘤恶性程度加重,Survivin与PTEN蛋白表达呈显著负相关;Survivin蛋白与cyclin D1蛋白表达之间呈显著正相关.结论:Survivin、PTEN及cyclin D1基因在肝细胞癌的发生、发展中起着不同的作用,联合检测Survivin和PTEN对判定肝细胞癌预后可提供一定的依据.

The Expression of Survivin in Human Hepatocellular Carcinoma and Its Correlation with PTEN and Cyclin D1

Objective: To investigate the expression of survivin and its relationship with the expression of PTEN and the cyclinD1. Methods: By flow cytometry (FCM) and immunofluorescence, the expression of survivin, PTEN and cyclinD1 were determined in 48 cases of hepatocellular carcinoma, 32 cases of paraneoplastic cirrhosis, 6 cases of cirrhosis, 6 cases of normal hepatic tissue. Results: The expressional level of survivin and cyclin D1 in tumor tissues were obviously higher than that in the paraneoplastic tissue, cirrhosis and normal hepatic tissue, but the expressional level of PTEN was much lower. The expression of survivin and PTEN was significantly correlated with the histological differentiation and prognosis, but not with the tumor size, the presence of portal vein thrombosis and the intrahepatic metastasis. With the progress of malignancy, significant correlation was observed between the survivin and the cyclinD1, while negative correlation was observed between the survivin and PTEN expression in hepatocellular carcinoma. Conclusion: This experiment sugguests that the survivin, the PTEN and the cyclin D1 may play different roles in the oncogenesis and progression of hepatocellular carcinoma. Joint detection of the survivin and PTEN gene products can supply certain evidence on prognosis of hepatocellular carcinoma.