HPLC法同时测定青叶胆中獐牙菜苦苷、龙胆苦苷和獐牙菜苷的含量

目的:建立青叶胆中獐牙菜苦苷、龙胆苦苷、獐牙菜苷的含量测定方法,为控制其质量提供科学依据。方法:采用高效液相色谱法。色谱条件:色谱柱为Phenomsil C18色谱柱(250 mm×4.6 mm,5μm);流动相为甲醇-0.02%磷酸溶液(23∶77);检测波长为243 nm;流速为1 mL/min;柱温为30℃。结果:3种成分均达到基线分离,獐牙菜苦苷、龙胆苦苷、獐牙菜苷的线性范围分别为0.33～1.98μg(r=0.9997),0.16～0.96μg(r=0.9999),0.20～1.20μg(r=0.9997),回收率分别为100.2%,99.5%,98.2%。结论:该法快速、准确、稳定,可用于青叶胆药材质量评价。

HPLC Simultaneous Determination of Swertiamarin Gentiopicroside and Sweroside in Swertia mileensis

Objective:To determinate the contents of the iridoids,swertiamarin,gentiopicroside and sweroside in Swertia mileensis.Methods:HPLC was used.The samples were separated on a phenomsil C18 column(250 mm×4.6 mm,5 μm)with which eluted with methanol and water(0.02% phosphoric acid).The ratio of methanol and water was 23:77.The gradient elution with flow rate was set at 1.0 mL/min,with UV detection at 243 nm and column temperature at 30 ℃.Results:Thee coilpounds were isolated on base line.The linear ranges of swertiamarin,gentiopicroside and sweroside were 0.33~1.98 μg(r=0.9997),0.16~0.96 μg(r=0.9997),0.20~1.20 μg(r=0.9997),respectively.The average recoveries were 100.2%,99.5% and 98.2%,respectively.Conclusion:The method has good reproducibility,and can be used in setting up the method to determinate the contents and quality control of Swertia mileensis from various habitats.