半夏泻心汤对幽门螺杆菌感染人胃上皮细胞GES-1生长的影响

目的:观察半夏泻心汤对幽门螺杆菌(Helicobacter pylori,Hp)感染的胃上皮细胞GES-1增殖的影响。方法:采用MTT法检测细胞的增殖。以细胞细菌不同比例体外共培养48 h,以确定两者的合适比例。用不同浓度的含药血清培养感染细胞。结果:细胞:细菌比例在1∶10以上时,Hp显著抑制细胞的增殖(P<0.05),而且呈量效关系;与模型组相比,半夏泻心汤能降低细菌对细胞增殖的抑制作用(P<0.05)。结论:Hp抑制细胞增殖,半夏泻心汤能扭转细菌的抑制作用。

Effect of Banxia Xiexin Decoction on Proliferation of Human Gastric Epithelial Line GES-1 Cells Infected by Helicobacter pylori

Objective:To investigate the effect of Banxia Xiexin Decoction on proliferation of GES-1 cells infected by Hp.Methods:Cell proliferation was determined by MTT assay.GES-1 cells were co-cultivated with Hp for 48 hours in vitro to determine the suitable proportion of cells to bacteria.The GES-1 cells infected by Hp were cultivated in different concentrations of drug-containing serum culture.Results:The GES-1 cells proliferation was significantly inhibited byHp in a dose-dependent manner at the rate of cells to bacteria over 1:10(P<0.05).The inhibition of GES-1 cells proliferation by Hp infecting was decreased by Banxia Xiexin Decoction-containing serum(P<0.05).Conclusion:Hp can inhibit the proliferation of GES-1 cells.Banxia Xiexin Decoction can reverse the inhibition.