脾气虚证自由基导致DNA损伤变化探析

目的：探讨脾气虚状态下模型大鼠体内DNA受到自由基攻击和氧化损伤的程度，了解脾气虚在中医脾病发展过程中的地位，进一步为脾气虚证提供实验学依据。方法：采用“饮食不节＋疲劳过度”复合方法造模14天后，取大鼠最后24h尿样测定8-OHdG的含量，取脑皮质和血清测定SOD、GSH—Px、T—AOC、MDA的含量。结果：脾气虚模型组大鼠血清和脑皮质中SOD、GSH—Px、T—AOC和MDA含量与正常组相比均有显著差异（P〈0．05）。模型组尿样中8-OHdG含量也有明显上调趋势（P〈0．05）。结论：脾气虚模型大鼠体内存在着明显的自由基损伤和抗氧化酶活性的降低，但脾气虚状态处于脾病全程的何种阶段尚需深入研究了解。

Research of DNA Damage Induced by Free Radicals in Syndrome of Spleen Qi Deficiency

Objective： This research was to explore the changing rule of DNA attacked by free radicals and oxidative damage in rats with spleen qi deficiency, understanding the role of spleen qi deficiency in the development process of spleen disease and providing experimental evidence for further research. Methods ： Fatty diet plus over fatigue method was used to establish the model for 14 days, and the last 24 h urine samples were taken to measure the content of 8-OHdG, and the SOD, GSH-Px, T-AOC and MDA contents were measured in brain cortex and serum. Results ： In serum and cerebral cortex, the contents of SOD, GSH-Px, T-AOC and MDA in the model group showed a significant difference （ P〈0.05 ） .The 8-OHdG in the urine samples of the model group was increased significantly （ P〈0.05 ） .Conclusion ： There is a clear reduction of antioxidant enzyme activities and free radical damage in spleen qi deficiency model, but the spleen oi deficiency state in which sta~e of the st~leen diseases is needed to be understood in-depth studies.