中草药专属性对照物质的研究

目的 :建立中草药专属性对照物质 (CSPD)用于其基原鉴定。方法 :选择具有代表性的 3种中草药 ,采用规范化的程序获取其专属性CSPD ;通过对不同来源的同种中草药CSPD的¹H-NMR图谱的比较分析 ,并对各中草药CSPD化学成分的分离和结构鉴定 ,实现对其整体化学组成的表达。结果 :3种具有代表性的中草药的CSPD的¹H-NMR图谱均有高度的特征性和重现性 ,并主要显示各中草药特征性成分的共振信号 ,与各中草药的基原严格相关。结论 :按照本研究操作程序 ,可以获得对多数中草药具有鉴别意义的专属性CSPD ,作为对其基原鉴定的参考。

Studies on the system for the exclusive control substance in traditional Chinese herbal medicines

Objective: To establish the system for the exclusive control substance of plant drug (CSPD) in traditional Chinese herbal medicines(TCHM) for the purpose of orginal identification. Method: Three kinds of representative TCHMs were selected and their exclusive CSPDs were obtained by standardized procedure. The expression of the total chemical characteristic of the CSPDs were achieved with the comparative analysis of the ¹H-NMR spectra for the CSPDs of the samples of the same species collected from different regions as well as the isolation and elucidation of the chemical constituents. Result: The ¹H-NMR spectra of the CSPDs for three kinds of representative TCHMs have highly characteristic features and reproducibility, and showed mainly the resonance signals of the characteristic constituents of each species. These ~1H-NMR spectra were strictly correlative with the origin of the species. Conclusion: By using the procedure of this paper, the exclusive CSPDs can be obtained for most of TCHMs, which have important significance and can be regarded as references in the original identification of TCHMs.