Cytokine-activated human endothelial cells synthesize and secrete a monocyte chemoattractant, MCP-1/JE. |
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Authors: | B J Rollins T Yoshimura E J Leonard and J S Pober |
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Institution: | Division of Medicine, Dana-Farber Cancer Institute, Boston, Massachusetts 02115. |
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Abstract: | We have demonstrated inducible expression of the mRNA encoding the monocyte chemoattractant MCP-1, the human homolog of the JE gene, in endothelial cells within 3 hours of treatment with IL-1 beta and tumor necrosis factor. IFN-gamma also induced expression of this mRNA after 24 hours, but to a lesser extent. MCP-1/JE protein steadily accumulated in the medium of endothelial cells during a 48-hour exposure to IL-1 beta. Medium conditioned by IL-1 beta-treated endothelial cells contained monocyte chemoattractant activity that was immunoadsorbed by anti-MCP-1 antibodies. These results suggest that endothelial cells secrete a monocyte chemoattractant, MCP-1/JE, in response to inflammatory mediators, and thus may contribute to the accumulation of monocytes at sites of inflammation. |
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