| Survivin在肾透明细胞癌786-O细胞株中表达的实验研究 |
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| 引用本文： | 李戈,王明.Survivin在肾透明细胞癌786-O细胞株中表达的实验研究[J].重庆医科大学学报,2010,35(1). |
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| 作者姓名： | 李戈 王明 |
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| 作者单位： | 重庆医科大学附属第一医院泌尿外科,重庆,400016;重庆医科大学附属第一医院泌尿外科,重庆,400016 |
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| 摘 要： | 目的:检测抗凋亡基因survivin在肾透明细胞癌786-O细胞株中的表达并探讨其临床意义.方法:运用MTT(四唑盐比色)法检测细胞生长状态并绘制生长曲线,运用RT-PCR(逆转录聚合酶链反应)法和免疫组化法检测786-O细胞株中survivinmRNA及survivin蛋白的表达情况.结果:检测出786-O细胞株中有survivin mRNA的表达,survivin蛋白的表达亦呈阳性,而正常肾细胞无阳性表达.结论:survivin mRNA及survivin蛋白在肾透明细胞癌中均有明显的表达,利用这一表达特异性,为以survivin基因为靶点的肾癌基因治疗研究提供了实验依据.
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| 关 键 词： | survivin 肾透明细胞癌 786-O RT-PCR 免疫组化 |
Experimental study on the expression of survivin in renal clear cell carcinoma 786-O |
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| LI Ge,et al.Experimental study on the expression of survivin in renal clear cell carcinoma 786-O[J].Journal of Chongqing Medical University,2010,35(1). |
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| Authors: | LI Ge |
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| Institution: | LI Ge,et al(Department of Urinary Surgery,the First Affiliated Hospital,Chongqing Medical University) |
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| Abstract: | Objective:To investigate the expression and the clinical significance of survivin in renal clear cell carcinoma 786-O.Methods:The expression of survivin mRNA and protein were detected by RT-PCR and immunohistochemisty,The growth curve of renal clear cell carcinoma 786-O were detected by MTT.Results:The survivin mRNA and protein were detected in 786-O cells.In contrast,no expression of survivin was found in normal renal cells.Conclusion:The survivin gene and protein were significantly expressed in renal clea... |
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| Keywords: | survivin 786-O RT-PCR Survivin Renal cell carcinoma 786-O RT-PCR Immunohistechemisty |
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