基质金属蛋白酶-8及组织抑制物-1与胎膜早破的关系

目的:探讨孕妇胎膜基质金属蛋白酶-8(MMP-8)及金属蛋白酶组织抑制物-1(TIMP-1)的水平变化以及与胎膜早破的关系。方法:30例胎膜早破孕妇作为胎膜早破组,其中妊娠37～41周18例,28～36+6周12例。30例非胎膜早破孕妇作为对照组,平均妊娠34～41+2周。应用免疫组织化学SP法和图像分析法检测两组孕妇胎膜MMP-8和TIMP-1水平,比较胎膜早破组与对照组在这些指标上的差异。结果:①胎膜早破组和对照组两组孕妇胎膜MMP-8的阳性表达分别为100%和70%,胎膜早破组水平明显高于对照组,差异有统计学意义(P<0.01);②胎膜早破组和对照组两组孕妇胎膜TIMP-1的表达,胎膜早破组低于对照组,但差异无统计学意义(P>0.05)。结论:MMP-8水平的异常升高及TIMP-1水平的下降,可能是胎膜早破重要的发病机制,MMP-8及TIMP-1的水平检测可作为一种新的生物学标志物用于胎膜早破并绒毛膜羊膜炎感染

Relationship between matrix metalloproteinase-8/tissue inhibitor of matrix metal loproteinase-1 and premature rupture of membranes

Objective:To explore the changes of matrix metalloproteinase 8(MMP-8) and tissue inhibitor of matrix metalloproteinase-1(TIMP-1) levels in fetal membranes of pregnant women and their relationship with premature rupture of membranes(PROM).Methods:30 pregnant women with PROM were enrolled in the study as PROM group(18 cases of 37～41 gestational weeks and 12 cases of 28～36+6 gestational weeks).30 pregnant women without PROM(34～41+2gestational weeks on average) were recruited as control group.MMP-8 and TIMP-1 levels in the two groups were detected by immunohistochemistry S-P method and computer image analysis system.Results:①The positive rates of MMP-8 in fetal membranes in PROM group and control group was 100% and 70%,respectively,there was significant difference(P<0.01).The expression rate of TIMP-1 in fetal membranes of PROM group was lower than that in control group without difference(P>0.05).Conclusion:It is suggested that PROM is associated with increased MMP-8 and decreased TIMP-1 levels.MMP-8 and TIMP-1 are valuable clinical biological markers for identifying PROM combined with chorioamnionitis.