孕酮对去卵巢大鼠的视神经保护作用

目的:用去卵巢大鼠建立更年期动物模型,给予不同浓度孕酮,检测血清中一氧化氮(NO)含量的变化,观察孕酮对视网膜神经节细胞凋亡影响。方法:用放射免疫分析方法(RIA)测定血清中孕酮浓度,用比色法测定血清中NO的含量;用TUNEL原位凋亡技术检测视网膜神经节细胞凋亡。结果:NO浓度随孕酮水平增加而升高,视网膜神经节细胞凋亡与孕酮含量呈负相关。结论:适量孕酮可能通过促进NO生成,从而抑制视网膜神经节细胞的凋亡,进而发挥其视神经保护作用。

Protective effect of progesterone on optic nerve of ovariectomized rats

Objective:To establish menopausal rat models with ovariectomized rats by injecting different levels of progesterone,detect the changes of nitric oxide level in serum,observe the effect of progesterone on apoptosis of retinal ganglial cells.Methods:The levels of progesterone and nitric oxide in serum were detected by RIA and colorimetry,respectively;the apoptosis of retinal ganglial cells was detected by TUNEL technique.Results:The level of nitric oxide increased with progesterone level,there was a negative correlation between apoptosis of retinal ganglial cells and progesterone level.Conclusion:Proper progesterone can inhibit the apoptosis of retinal ganglial cells by promoting the production of nitric oxide,in order to protect optic nerve.