Delayed nerve repair increases number of caspase 3 stained Schwann cells |
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Authors: | Harukazu Saito Martin Kanje Lars B Dahlin |
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Institution: | 1. Department of Orthopaedic Surgery, Murayama Medical Center, National Hospital Organization, Tokyo, Japan;2. Department of Cell and Organism Biology, Lund University, Lund, Sweden;3. Department of Hand Surgery, Malmö University Hospital, SE-205 02 Malmö, Sweden |
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Abstract: | Caspase 3 staining in Schwann cells was investigated with immunohistochemistry, as a measure of Schwann cell apoptosis, after transection and immediate (day 0) or delayed rat sciatic nerve repair (30, 90 and 180 days post injury). Cleaved caspase 3 stained Schwann cells significantly increased at the site of lesion (SNL; median IQR], 15.2 7.0] %) and in the distal nerve segment (SND; 9.5 3.6] %) 10 days after immediate repair. The number of cleaved caspase stained Schwann cells also increased significantly after delayed repair, irrespective of length of delay, at both locations (SNL: 22.0–27.1%; SND: 18.5–22.1%; p < 0.05). Some cleaved caspase 3 stained satellite cells were seen in dorsal root ganglia on the injured side, but no stained motor or sensory neurons were observed at any time-point. Delayed nerve repair is associated with more pronounced Schwann cell apoptosis which may explain impaired nerve regeneration after nerve injury and delayed repair. |
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Keywords: | Schwann cells Cleaved caspase 3 Apoptosis Nerve repair Satellite cells |
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