Down regulation of TRPC1 by shRNA reduces mechanosensitivity in mouse dorsal root ganglion neurons in vitro |
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Authors: | Susanne Staaf Ingela Maxvall Ulrika Lind Johanna Husmark Jan P. Mattsson Patrik Ernfors Stefan Pierrou |
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Affiliation: | 1. Department of Bioscience, AstraZeneca R&D Mölndal, 431 83 Mölndal, Sweden;2. Division of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 171 77 Stockholm, Sweden;3. Department of Cell and Molecular Biology, University of Gothenburg, 41390 Gothenburg, Sweden;4. Albireo AB, 430 33 Göteborg, Sweden |
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Abstract: | Mechanosensitivity is a crucial but poorly understood property of the sensory nervous system. Transient receptor potential (TRP) channels, which have been found to be responsible for the detection of other sensory stimuli such as temperature and pungent chemicals, have been suggested to also recognize stretch or pressure to cell membranes. TRPC1 is one candidate from studies in oocytes but evidence in native sensory neurons has been lacking. Therefore, we have measured an increase in intracellular Ca2+ levels upon mechanical activation of native mouse dorsal root ganglion (DRG) neurons in culture using hypoosmolar buffer. Our results show that down regulation of TRPC1 with short hairpin RNA results in a 65% reduction of neurons with stretch activated responses. These results implicate a direct or indirect involvement of TRPC1 in the mechanosensitivity of DRG neurons. |
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Keywords: | Transient receptor potential channels Dorsal root ganglion Mechanosensitivity Sensory neuron |
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