Effect of portal hypertension onIn Vivo bile acid-mediated small intestinal mucosal injury in the rat |
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Authors: | Dr. Gavin Jonas MD Richard A. Erickson MD Timothy Morgan MD |
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Affiliation: | (1) Department of Medicine, Veterans Administration Medical Center, 90822 Long Beach;(2) University of California, 92717 Irvine, California;(3) Gastroenterology Section, Medical Service (111G), Veterans Administration Medical Center, 5901 East Seventh Street, 90822 Long Beach, California |
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Abstract: | This study's purpose was to determine whether portal hypertension adversely affects small intestinal mucosal injury. Portal hypertension was produced in male Sprague-Dawley rats by two-stage ligation of the portal vein. Sham-operated rats were used as controls. Two weeks later, intestinal injury was produced byin vivo perfusion with 5 mM chenodeoxycholic acid for 30 min. Intestinal injury was assessed by quantitative morphometry and by measuring intestinal water and mannitol absorption. Portal hypertension resulted in more injury in the distal perfused intestine as manifested by increased villus tip denudation [portal hypertensive 52.5±9.6sem) vs controls 28.1±5.7m, P=0.05). Additionally there was a significant decrease in the unperfused duodenal villus height in portal hypertensive rats (portal hypertensive 755±22 vs controls 848±28m, P<0.02). Portal hypertension had no significant effect on the increase in mannitol absorption or water secretion caused by chenodeoxycholic acid perfusion. This study suggests that portal hypertension alters small intestinal mucosa and increases susceptibility to injury.This work was supported in part by a grant from the Research Service of the Veterans Administration. |
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Keywords: | portal hypertension bile acid chenodeoxycholic acid rats small intestine quantitative morphometry |
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