| 莲心碱对自发性高血压大鼠血管平滑肌细胞增殖及对PDGF-B、bFGF、c-sis、c-myc的影响 |
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| 引用本文： | 熊一力,王嘉陵,钱家庆.莲心碱对自发性高血压大鼠血管平滑肌细胞增殖及对PDGF-B、bFGF、c-sis、c-myc的影响[J].中国心血管杂志,1998(1). |
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| 作者姓名： | 熊一力 王嘉陵 钱家庆 |
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| 作者单位： | 同济医科大学同济医院 武汉430022
(熊一力，王嘉陵)，同济医科大学同济医院 武汉430022(钱家庆) |
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| 基金项目： | 国家卫生部科研基金，国家教委优秀年轻教师基金 |
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| 摘 要： | 用~3H]胸腺嘧定核苷(~3H]TdR)掺入法、电镜、免疫组化和原位杂交方法,在自发性高血压大鼠(SHR)观察了莲心碱(Lien)对血管平滑肌细胞(VSMC)增殖的作用及对生长因子PDGFB、bFGF及其相关癌基因c-sis和c-myc表达的影响.结果发现Lien在降低SHR血压同时,能减少VSMC的线粒体,粗面内质网和~3H]TdR掺入量,并能逆转VSMC增殖时PDGF-B、bFGF抗原及c-sis和c-myc mRNA的表达增强.提示Lien能抑制SHR的VSMC增殖,与生长因子及癌基因调控的分子生物学机制有关.
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| 关 键 词： | 莲心碱 自发性高血压大鼠 肌 平滑 血管 生长因子 癌基因 |
Effect of liensinine on proliferation of vascular smooth muscle cells and PDGF-B, bFGF, c-sis and c-myc in spontaneously hypertensive rat. |
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| Xiong Yili,Wang Jialin,Qian Jiaqing.Effect of liensinine on proliferation of vascular smooth muscle cells and PDGF-B, bFGF, c-sis and c-myc in spontaneously hypertensive rat.[J].Chinese Journal of Cardiovascular Medicine,1998(1). |
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| Authors: | Xiong Yili Wang Jialin Qian Jiaqing |
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| Institution: | Xiong Yili,Wang Jialin,Qian Jiaqing. Department of Medicine,Tongji Hospital,Wuhan 430022. |
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| Abstract: | In order to determine the effects of Liensinine on proliferation of vascular smooth muscle cells (VSMC) and expression growth factor PDGF-B,bFGF and oncogene c-sis, c-myc in spontaneously hypertensive rat (SHR), 3H]thymidine(3H]TdR)incorporation,electronic microscopy, immunohistochemistry and in situ hybridization assaies were used. The results showed that after administration of Liensinine, morphological change of VSMC was antagnized,blood presure and 3H]TdR data were significantly reduced. Furthermore, Liensinine could reverse the enhanced expression of antigen PDGF-B, bFGF and reduced expression of oncogene c-sis and c-myc in SHR. These results suggested that Liensinine might inhibit proliferation of VSMC in SHR, related with the mechanism of molecular biology of controlling growth factors and oncogene. |
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| Keywords: | Liensinine spontaneously hypertensive rat muscle smooth vascular growth fac- tor oncogene |
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