Serum transfer RNA‐derived fragment tRF‐31‐79MP9P9NH57SD acts as a novel diagnostic biomarker for non‐small cell lung cancer |
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| Authors: | Jipeng Li Chao Cao Laifu Fang Wanjun Yu |
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| Affiliation: | 1. Department of Central Laboratory, The Affiliated People''s Hospital, Ningbo University, Ningbo China ; 2. Department of Respiratory and Critical Medicine, Ningbo First Hospital, Ningbo China ; 3. Department of Pathology, The Affiliated People''s Hospital, Ningbo University, Ningbo China ; 4. Department of Respiratory and Critical Medicine, The Affiliated People''s Hospital, Ningbo University, Ningbo China |
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| Abstract: | BackgroundtRNA‐derived fragments (tRFs) have been found to have a crucial function in the pathophysiology of cancers. However, the function of tRFs in non‐small cell lung cancer (NSCLC) is yet unknown. The goal of this study was to assess the tRF‐31‐79MP9P9NH57SD serum expression from NSCLC patients and to determine its diagnostic usefulness.MethodsBy using stem‐loop quantitative real‐time PCR, we were able to detect various tRF‐31‐79MP9P9NH57SD expressions in 96 NSCLC serum samples, 96 healthy controls, and 20 pairs of NSCLC serum samples pre‐ and post‐surgery (qRT‐PCR). After that, we analyzed its diagnostic effectiveness using the receiver operating characteristic (ROC) curve.ResultsSerum tRF‐31‐79MP9P9NH57SD expression was higher in NSCLC patients, and levels of tRF‐31‐79MP9P9NH57SD were linked to the clinical stage (p = 0.002) and the malignancy of lymph node (p = 0.012). In addition, after the procedure, the serum tRF‐31‐79MP9P9NH57SD expression in NSCLC patients dropped. With 48.96 percent sensitivity and 90.62 percent specificity, the area under ROC curve (AUC) was 0.733.Conclusionserum tRF‐31‐79MP9P9NH57SD possibly is a new and groundbreaking biomarker for the NSCLC. |
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| Keywords: | biomarker, NSCLC, tRF‐ 31‐ 79MP9P9NH57SD, tRNA‐ derived small RNAs |
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