A novel metal–organic framework of Ba–hemin with enhanced cascade activity for sensitive glucose detection |
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Authors: | Jintao Yi Xianqin Han Fengying Gao Le Cai Ying Chen Xiulong Deng Xun Li Jun Xue Hui Zhou |
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Affiliation: | Key Laboratory of Organo-Pharmaceutical Chemistry of Jiangxi Province, Gannan Normal University, Ganzhou 341000 P. R. China, Fax: +86-797-8393536, +86-797-8393536 ; Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, School of Pharmacy, Guangdong Medical University, Dongguan 523808 P. R. China |
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Abstract: | Early glucose detection is important in both healthy people and diabetic patients. Glucose biosensing based on glucose oxidase (GOX) is a common method. However, native proteins are mostly membrane impermeable and are prone to degradation in complex sample environments. Herein, we report a facile one-step biomineralization method by simply mixing aqueous solutions of hemin and barium nitrate with glucose oxidase (GOX) to form Ba–hemin@GOX composites. Glucose (Glu) is introduced through self-driven sampling to trigger the GOX-catalysed production of hydrogen peroxide, which could help the subsequent 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation reaction catalysed by Ba–hemin to yield the blue-coloured product. The sensor exhibited a detection limit as low as 3.08 μM. The operability and accuracy of the Ba–hemin@GOX biosensor were confirmed by the quantitative determination of glucose in real samples, such as tap water, serum and drinks. Moreover, the Ba–hemin@GOX-based colorimetric biosensor showed good selectivity, storage stability and recoverability. The experimental results reveal that a GOX activity of more than 90% was still maintained even after being incubated at 60 °C for 30 minutes, and Ba–hemin@GOX could be reused for glucose detection at least six times. Even after 30 days of storage, the relative activity was still more than 90%. Overall, the developed Ba–hemin@GOX biosensor provides a valuable and general platform for applications in colorimetric biosensing and medical diagnostics.The Ba–hemin@GOX composite is used for sensitive glucose detection. |
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