| 小鼠T-bet基因真核表达载体的构建 |
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| 引用本文： | 檀卫平,麦友刚,黄嘉凌,刘然义,麦贤弟,黄绍良,黄文林. 小鼠T-bet基因真核表达载体的构建[J]. 广东医学, 2005, 26(10): 1332-1334 |
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| 作者姓名： | 檀卫平 麦友刚 黄嘉凌 刘然义 麦贤弟 黄绍良 黄文林 |
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| 作者单位： | 中山大学附属第二医院儿科,广州,510120;中山大学肿瘤防治中心,广州,510060 |
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| 基金项目： | 广东省自然科学基金资助项目(编号:031668A) |
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| 摘 要： | 目的构建小鼠T—bet基因重组真核表达载体pcDNA3一T—bet,为T—bet基因治疗研究提供有效的生物表达系统。方法采用内切酶切从质粒T—bet/GFP—RV中获得约1.7 kb的小鼠T—betcDNA片段,以单酶切非定向克隆方式与真核表达质粒pcDNA3连接,构建真核表达载体pcDNA3-T-bet。结果经PCR筛选阳性重组子,双酶切鉴定T—bet基因重组方向,并测序鉴定无错配及插入移位等DNA顺序改变。结论本实验成功构建了小鼠T—bet基因真核表达载体pcDNA3-T-bet。
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| 关 键 词： | T-bet基因 真核表达载体pcDNA3-T-bet 小鼠 |
| 收稿时间： | 2005-03-16 |
| 修稿时间： | 2005-03-16 |
Construction of murine T -bet cDNA eukaryotic expression vectors |
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| Tan Weiping , Mai Yougang , Hang Jialing. Construction of murine T -bet cDNA eukaryotic expression vectors[J]. Guangdong Medical Journal, 2005, 26(10): 1332-1334 |
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| Authors: | Tan Weiping Mai Yougang Hang Jialing |
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| Affiliation: | Department of Pediatrics, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120 |
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| Abstract: | Objective To construct murine T- betcDNA eukaryotic expression vectors pcDNA3 - T- bet for facilitating the study of T- bet gene therapy. Methods An approximately 1.7 kb murine T- betcDNA segment was excised from plasmid T - bet/GFP- RV. T- betcDNA was ligated with pcDNA3 in Xho I enzyme site named as pcDNA3 - T- bet. Results The recombiant plasmids was identified by PCR amplification and restriction enzymes map. Conclusion The murine T- betcDNA eukaryotic expression vector pcDNA3 - mT- bet is successfully constructed in this experiment. |
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| Keywords: | T-bet gene Eukaiyotic expression vectors pcDNA3 - mT- bet Murine |
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