高效液相色谱法同时测定赤丹丸中6种有效成分的含量

 目的建立高效液相色谱法测定赤丹丸中丹参素钠、黄芩苷、黄芩素、汉黄芩素、汉黄芩苷和丹参酮ⅡA含量的方法。方法Agilent Zorbax Extend-C₁₈(4.6mm×250mm,5μm)柱;流动相组成为A:甲醇,B:水(磷酸调pH3.0),C:乙腈,进行梯度洗脱;流速1.0mL·min^-1;检测波长280nm。结果丹参素钠线性范围16.4～327.0mg·L^-1,平均回收率为97.5%,RSD为1.31%;黄芩苷线性范围15.8～316.0mg·L^-1,平均回收率为97.4%,RSD为1.02%;黄芩素线性范围10.3～206.0mg·L^-1,平均回收率为99.3%,RSD为0.68%;汉黄芩素线性范围12.6～251.0mg·L^-1,平均回收率为100.2%,RSD为1.76%;汉黄芩苷线性范围11.3～225.0mg·L^-1,平均回收率为99.4%,RSD为2.6%;丹参酮ⅡA线性范围5.04～100.7mg·L^-1,平均回收率为97.8%,RSD为1.4%;结论本方法测定结果准确可靠,可用于测定赤丹丸中6成分的含量。

Simultaneous Determination of Sodium Salvianic A Baicalin, Baicalein, Wogonin, Wogonoside and Tanshinone Ⅱ A in Chidan Pills by HPLC

OBJECTIVE To develop a quantitative method for the determination of sodium salvianic A,baicalin,baicalein,wogonin,wogonoside and tanshinoneⅡA in Chidan pills by HPLC.METHODS A column of Agilent Zorbax Extend-C₁₈(4.6 mm×250 mm,5 μm) was used.The mobile phase composed of methanol(A),water adjusted to pH 3.0 with phosphoric acid(B) and acetonitrile(C) with gradient elution.The flow rate was 1.0 mL·min^-1 with UV detection wavelength at 280 nm.RESULTS The linear range of sodium salvianic A was 16.4～327.0 mg·L^-1.The linear range of baicalin was 15.8～316.0 mg·L^-1.The linear range of baicalein was 10.3～206.0 mg·L^-1.The linear rang of wogonin was 12.6～251.0 mg·L^-1.The linear range of wogonoside was 11.3～225.0 mg·L^-1 and the linear range of tanshinoneⅡA was 5.04～100.7 mg·L^-1.The average recovery of sodium salvianic A,baicalin,baicalein,wogonin,wogonoside and tanshinoneⅡA were 97.5%(RSD=1.31%),97.4%(RSD=1.02%),99.3%(RSD=0.68%),100.2%(RSD=1.76%),99.4%(RSD=2.6%) and 97.8%(RSD=1.4%),respectively.CONCLUSION The method is sensitive,rapid and accurate.