ICP-MS同时分析中药材中7种微量元素的方法研究

 目的建立用电感耦合等离子体质谱(ICP-MS)同时分析中药材中铜(Cu)、砷(As)、镉(Cd)、汞(Hg)、铅(Pb)、硒(Se)、锗(Ge)7种元素的方法。方法样品经微波消解后,以钪(⁴⁵Sc)、钇(⁸⁹Y)、铟(¹¹⁵In)、铋(²⁰⁹Bi)作为内标物质,直接用ICP-MS分析上述7种元素,并通过标准曲线计算含量。结果7种元素标准曲线的线性关系良好,相关系数(r)在0.999 3～0.999 9之间;检出限0.019～0.107μg·L^-1;加样回收率88.92%～115.0%;5次平行实验的分析结果在3 d内稳定性良好,且所有元素的相对标准偏差(RSD)均小于10%;除砷(As)略低外,标准物质茶叶(GBW 07605)中其他元素的测定值都在标准值范围内。结论该方法是一个灵敏、可靠、稳定的中药材多元素分析方法。

Determination of 7 Trace Elements in Traditional Chinese Medicine by ICP-MS

OBJECTIVE To establish a method for simultaneous analysis of 7 elements(Cu,As,Cd,Hg,Pb,Se,Ge) in Traditional Chinese Medicines(TCM) by Inductively Coupled Plasma Mass Spectrometry(ICP-MS).METHODS With ⁴⁵Sc,⁸⁹Y,¹¹⁵In and ²⁰⁹Bi as internal standard elements,the sample solutions were analyzed by ICP-MS after microwave digestion,and the contents of elements were calculated according to their calibration curves.RESULTS The correlative coefficients of calibration curve for all elements were higher than 0.099 3 and the limits of detection were between 0.019 μg·L^-1 and 0.107 μg·L^-1.The analytical values of parallel tests of samples(n=5) were stable in three days and the relative standard deviations for all elements were lower than 10%.The average spiked recoveries ranged from 88.92% to 115.0% and the analytical results of tea SRM(GBW 07605) showed acceptable agreement with the certified values.CONCLUSION This method is a sensitive,reliable and stable method for the multi-elements determination of Traditional Chinese Medicine by ICP-MS.