反相高效液相色谱法同时测定血浆中10种苯二氮类药物

 目的建立反相高效液相色谱法同时测定人血浆中10种苯二氮类药物(氯氮、咪达唑仑、硝西泮、奥沙西泮、艾司唑仑、劳拉西泮、阿普唑仑、氯硝西泮、地西泮和三唑仑)。方法血浆样品经二氯甲烷萃取后,采用Shim-pack CLC-C₈色谱柱,柱温35℃,流动相为0.01mol·L^-1磷酸盐缓冲液-乙腈-无水乙醇-甲醇(68.2∶31.2∶0.3∶0.3),流速为1.10mL·min^-1,紫外检测波长230nm。结果血浆中氯氮、咪达唑仑、硝西泮、奥沙西泮、艾司唑仑、劳拉西泮、阿普唑仑、氯硝西泮、三唑仑和地西泮在40～2000μg·L^-1内线性关系良好(r≥0.9992),平均方法回收率均在97%～104%,萃取回收率均在70%以上,日内和日间RSD均≤11%(n=5)。结论本方法准确快速、简便易行,适用于人血浆中10种苯二氮类药物的同时检测。

Simultaneous Determination of Ten Benzodiazepines in Human Plasma by RP-HPLC

OBJECTIVE To establish a HPLC method for simultaneous determination of frequently prescribed 10 benzodiazepines(chlordiazepoxide,midazolam,nitrazepam,oxazepa,estazolam,lorazepam,alprazolam,clonazepam,diazepam and triazolam)in human plasma.METHODS The benzodiazepines were extracted with dichloromethane from plasma samples,and then was analyzed on a Shim-pack CLC-C₈ column(6.0 mm×150 mm,5 μm)at 35 ℃ with the mobile phase consisting of 0.01 mol·L^-1 phosphate buffer solution-acetonitrile-ethanol-methanol(68.2∶31.2∶0.3∶0.3),at a flow rate of 1.10 mL·min^-1.Ultraviolet detection wavelength was at 230 nm.RESULTS The linear ranges were 40～2 000 μg·L^-1(r≥0.999 2).The average recovery of 10 drugs were within 97%～104%.The average extraction recoveries were all over 70%.Intra-day RSD and inter-day RSD were less than 11%(n=5).CONCLUSION The method is accurate,rapid and convenient for simultaneous determination of 10 benzodiazepines in human plasma.