庆大霉素毫微球的体内抗菌活性研究

 目的：观察庆大霉素与聚氰基丙烯酸正丁酯毫微球结合以后，其体内抗菌活性(特别是对细胞内感染)是否有所增加。方法：以鼠伤寒沙门杆菌感染的C57BL/6j小鼠为细胞内感染的动物模型，以小鼠存活率和器官组织中的活菌计数为指标，对庆大霉素聚氰基丙烯酸正丁酯毫微球的体内抗菌活性进行评价。结果：从存活率评价其治疗指数提高了10倍。肝、脾、肾中活菌计数最低可降至庆大霉素溶液组的1/426，1/141和1/30。结论：与庆大霉素溶液相比，庆大霉素毫微球明显提高了对伤寒沙门杆菌感染小鼠的治疗效果。

In vivo antimicrobial activity of gentamicin polybutylcyanoacrylate nanoparticles

To observe whether the in vivo antimicrobial activity of gentamicin (specially in the intracellular infected animals) could be improved after associting with polybutylcyanoacrylate nanoparticles. METHOD: S. typhimurium infected C57BL/6j mice were chosen as the animal model of the intracellular infection, then the antimicrobial activity of gentamicin polycyano-eacrylate nanoparticles (GM-NP) was evaluated by the mouse survival and the bacterial counts. RESULTS: By comparison with gentamicin solution (GM-Sol), the therapeutic index of GM-NP based on mouse mortality was incrased by 10 fold, and the bacterial counts on liver, spleen and kidney could be as low as 1/426, 1/141 and 1/30 of GM-Sol treated groups respectively. CONCLUSION: By comparison with GM-Sol, in vivo antimicrobial activity of gentamicin over S. typhimurium infected C57BL/6j mice improved significantly after associting with polybutylcyanoacrylate nanoparticles.