| 高效液相色谱法测定金钱草颗粒中槲皮素含量 |
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| 引用本文： | 谭忠谋,韦建乔,蒙露露.高效液相色谱法测定金钱草颗粒中槲皮素含量[J].中国药业,2012,21(9):24-25. |
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| 作者姓名： | 谭忠谋 韦建乔 蒙露露 |
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| 作者单位： | 广西壮族自治区河池市食品药品检验所,广西河池,574000 |
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| 摘 要： | 目的建立测定金钱草颗粒中槲皮素含量的高效液相色谱法。方法色谱柱为Phenomenex Prodigy ODS3 C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-0.4%磷酸溶液(50∶50),流速为1.0 mL/min,检测波长为360 nm,柱温为30℃。结果槲皮素质量浓度在10.4～104.0μg/mL范围内与峰面积呈良好线性关系(r=0.999 9),平均回收率为99.92%,RSD=1.07%(n=6)。结论该方法简便、准确、重复性好,可用于测定金钱草颗粒的含量。
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| 关 键 词： | 含量测定 金钱草颗粒 槲皮素 高效液相色谱法 |
Content Determination of Quercetin in Lysimachia Granules by HPLC |
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| Tan Zhongmou
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Wei Jianqiao
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Mong Lulu.Content Determination of Quercetin in Lysimachia Granules by HPLC[J].China Pharmaceuticals,2012,21(9):24-25. |
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| Authors: | Tan Zhongmou Wei Jianqiao Mong Lulu |
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| Institution: | Tan Zhongmou,Wei Jianqiao,Mong Lulu(Hechi Institute for Food and Drug Control,Hechi,Guangxi,China 574000) |
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| Abstract: | Objective To establish an HPLC method for determining the content of quercetin in Lysimachia Granules.Methods HPLC method was used.The column type wa s Phenomenex Prodigy ODS3(250 mm×4.6 mm,5 μm).The mobile phase was methanol0.4%phosphorous acid(50 ∶50).The flow rate was 1 mL/min.The detective waveleng th was 360 nm and column tempreture was 30 ℃.Results The linear range of querce tin was 10.4-104.0 μg/mL(r=0.999 9),the average recovery was 99.92%and the RS D was 1.07%(n=6).Conclusion The method is simple and accurate,and can be used reliably for the quality control of Lysimachia Granules. |
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| Keywords: | content determination Lysimachia Granules quercetin HPLC |
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