基质金属蛋白酶-9与缺血修饰白蛋白对冠心病的诊断价值

目的探讨基质金属蛋白酶-9与缺血修饰白蛋白的联合检测对冠心病的诊断价值和临床意义。方法随机选取体检健康者50例(A组),选取冠心病患者116例,其中稳定型心绞痛患者40例(B组),不稳定型心绞痛患者46例(C组),急性心肌梗死患者30例(D组)。采用酶联免疫吸附法(ELISA)测定各组血清基质金属蛋白酶-9(MMP-9)浓度,采用白蛋白结合钴(ACB)实验测定各组缺血修饰白蛋白(IMA)浓度,分别比较各组MMP-9和IMA水平,并进行相关性分析。结果 D组患者血清MMP-9和IMA浓度显著高于A、B组(P<0.01),D组患者血清MMP-9和IMA浓度明显高于C组(P<0.05);C组患者血清MMP-9和IMA浓度明显高于A、B组(P<0.05);B组患者血清MMP-9和IMA浓度高于A组,但差异无统计学意义(P>0.05)。急性冠脉综合征患者血清MMP-9和IMA水平成正相关(r=0.485,P<0.05)。结论 MMP-9与IMA是急性冠脉综合征的早期敏感指标,二者的联合检测可能是预测冠心病危险程度的重要指标。

Value of matrix metalloproteinase-9 and ischemia-modified albumin for diagnosis of coronary heart disease

Objective To explore the value of MMP-9 and IMA for diagnosis of coronary heart disease and its clinical significance.Methods Totally 50 cases in physical examination(group A),116 patients with coronary heart disease were randomly selected.Then divided patients with coronary heart disease into 40 subjects with SAP(group B),46 subjects with UAP(group C) and 30 subjects with AMI(group D).The concentrations of serum MMP-9 were detected with enzyme-linked immunosorbent assay(ELISA).And concentrations of ischemia-modified albumim(IMA) were detected with albumin cobalt binding(ACB) test.Four groups were separately compared the levels of MMP-9,IMA and to analysis the correlativity between the two.Results The levels of serum MMP-9 and IMA in group D were obviously higher than those of group A and B(P<0.01).The levels of serum MMP-9 and IMA in group D were significantly higher than those of group C(P<0.05).The levels of serum MMP-9 and IMA in group C were significantly higher than those of group A and B(P<0.05).The leves of serum MMP-9 and IMA in group B were higher than those of group A,but it was no significant(P>0.05).MMP-9 leves correlated positively with IMA leves in ACS patients(P<0.05).Conclusions MMP-9 and IMA were early sensitive maker for ACS.The joint measurement of MMP-9 and IMA may be the important maker to forecast the dangerous grade of CHD.