| 基质相互作用分子1基因敲除抑制血管平滑肌细胞增殖 |
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| 引用本文： | 郭瑞威,黄岚,宋明宝,王红,李魏. 基质相互作用分子1基因敲除抑制血管平滑肌细胞增殖[J]. 高血压杂志, 2008, 0(11) |
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| 作者姓名： | 郭瑞威 黄岚 宋明宝 王红 李魏 |
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| 作者单位： | 第三军医大学附属新桥医院全军心血管研究所; |
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| 摘 要： | 目的探讨基质相互作用分子1(STIM1)基因敲除对血管平滑肌细胞内钙浓度和细胞增殖的调控作用。方法原代培养血管平滑肌细胞,用构建好的大鼠 STIM1干扰腺病毒载体(Ad-si/rSTIM1)和人重组 ST1M1腺病毒载体(Ad-hSTIM1)进行转染,Western blot 检测细胞 STIM1表达,~3H 胸腺嘧啶核苷(~3H-TdR)掺入法和细胞计数测定细胞增殖,激光共聚焦检测细胞内钙变化。结果 Ad-si/rSTIM1转染后48h 后细胞 STIMl表达与转染对照腺病毒载体比较明显降低(P<0.05),细胞内钙浓度也明显下降(P<0.05),细胞增殖被显著抑制(P<0.05);Ad-hSTIM1共转染后48 h 细胞 STIM1表达恢复到正常水平;细胞内钙浓度和细胞增殖恢复到正常水平。结论 STIM1调节血管平滑肌细胞内钙浓度,并进而参与对血管平滑肌细胞增殖的调控。
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| 关 键 词： | 血管平滑肌细胞 基质相互作用分子1 细胞增殖 细胞内钙 RNAi |
Stromal Interaction molecule 1 STIM1 Knockdown Inhibits Proliferation of Vascular Smooth Muscle Cells |
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| GUO Rui- wei HUANG Lan SONG Ming-bao WANG Hong LI Wei. Cardiovascular Research Institute of Xinqiao Hospital,Third Military Medical University,Chongqing ,China. Stromal Interaction molecule 1 STIM1 Knockdown Inhibits Proliferation of Vascular Smooth Muscle Cells[J]. Chinese Journal of Hypertension, 2008, 0(11) |
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| Authors: | GUO Rui- wei HUANG Lan SONG Ming-bao WANG Hong LI Wei. Cardiovascular Research Institute of Xinqiao Hospital Third Military Medical University Chongqing China |
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| Affiliation: | GUO Rui- wei HUANG Lan SONG Ming-bao WANG Hong LI Wei. Cardiovascular Research Institute of Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China |
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| Abstract: | Objective To explore the role of stromal interaction molecule 1(STIMI)on prohteration and intra- cellular Ca~(2+)change in vascular smooth muscle ceils(VSMC).Methods Rat VSMC were isolated from SD rats and primary cultured.Ad-si/rSTIM1 and Ad-hSTIM1 were transfected into VSMC.The protein of STIM1 was measured by Western blot,the proliferation of VSMC was analyzed by ~3H-thymidine(~3H-TdR)incorporation and cell count,the intracellular Ca~(2+)change was assessed By Aquaeosmos system.Ruselts Fourty-eight hours... |
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| Keywords: | Vascular smooth muscle cell Stromal interaction molecule 1 Cell proliferation Intracellu lar Ca~(2+) RNAi |
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