左旋甲羟戊酸对辛伐他汀调控心肌细胞PTEN表达的影响 |
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引用本文: | 陈永清,赵连友,张卫泽,马凌. 左旋甲羟戊酸对辛伐他汀调控心肌细胞PTEN表达的影响[J]. 西北国防医学杂志, 2011, 32(2): 100-102 |
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作者姓名: | 陈永清 赵连友 张卫泽 马凌 |
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作者单位: | 1. 兰州军区兰州总医院心内科,甘肃,兰州,730050 2. 第四军医大学唐都医院心内科 |
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摘 要: | 目的:观察左旋甲羟戊酸(L-MVA)对辛伐他汀调控大鼠心肌细胞第十号染色体缺失的张力蛋白同源区(PTEN)表达的影响。方法:应用图像分析及[3H]-亮氨酸掺入法测定细胞表面积及蛋白合成速率,RT-PCR检测心钠素(ANP)mRNA表达,RT-PCR和Western blot法检测PTEN的mRNA和蛋白表达水平。结果:辛伐他汀+L-MVA组心肌细胞表面积、[3H]-亮氨酸掺入率、ANP mRNA水平均明显高于辛伐他汀组(P〈0.01),而PTEN的mRNA及蛋白表达水平则明显降低(P〈0.01)。结论:L-MVA能够降低PTEN的表达水平,逆转辛伐他汀对心肌细胞肥大的抑制作用。
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关 键 词: | 左旋甲羟戊酸 辛伐他汀 心肌细胞 肥大 PTEN |
Modulation of L-mevalonate on the effects of simvastatin on PTEN expression of cardiac myocytes in rats |
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Affiliation: | CHEN Yong-qing,ZHAO Lian-you,ZHANG Wei-ze,et al.(Department of Cardiology,Lanzhou General Hospital,Lanzhou Command,PLA,Lanzhou 730050,China) |
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Abstract: | Objective:To study the modulation of L-mevalonate(L-MVA) on the effects of simvastatin on PTEN expression in cultured rat cardiac myocytes.Methods: Image analysis system and -leucine incorporation were used to measure cell surface area and protein synthesis.The mRNA expression of atrial natriuretic peptide(ANP)was determined with RT-PCR.RT-PCR and Western blot were applied to study the mRNA and protein expression of PTEN,respectively.Results: Cell surface area,-leucine incorporation rate,ANP mRNA levels in simvastatin+L-MVA group were significantly higher than those of simvastatin group(P<0.01),while the mRNA and protein levels of PTEN in simvastatin+L-MVA group were significantly lower than those of simvastatin group(P<0.01).Conclusion:L-MVA can decrease the expression level of PTEN,thus reverse the inhibition effects of simvastatin on cardiac myocytes hypertrophy. |
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Keywords: | PTEN |
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