灯盏细辛总咖啡酸酯对TNF-α诱导脑微血管内皮细胞ICAM-1表达的影响

目的:研究灯盏细辛中总咖啡酸酯(total Caffeoyl quinic acid ester,Caf)对离体培养的大鼠脑微血管内皮细胞(brain microvascular endothelial cell,BMEC)细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)基因与蛋白表达的影响,初步探讨Caf抗脑缺血炎症损伤的机制。方法:采用肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)建立离体培养的大鼠BMEC炎症损伤模型,选用25μg/mL Caf处理内皮细胞,利用Real time RT-PCR方法检测实验各组ICAM-1mRNA表达,流式细胞仪测定实验各组ICAM-1蛋白荧光强度。结果:Caf可以下调TNF-α诱导的内皮细胞ICAM-1mRNA高表达,并抑制TNF-α诱导的内皮细胞膜ICAM-1蛋白高表达。结论:Caf可以降低由TNF-α诱导的内皮细胞过度表达ICAM-1,可能是其发挥抗脑缺血炎症损伤的部分机制。

Effect of Total Caffeoyl Quinic Acid Ester on Expression of Inflammatory Molecules Induced by TNF-α in Endothelial Cells

Objective：To investigate the effect of total Caffeoyl quinic acid ester（Car）on the expression of intercellular adhesion molecule-1 （ICAM-1）in rat brain miemvaseular endothelial cells（BMEC） cultivated in vitro. Methods：The inflammatory injury model was induced by tumor necrosis factor-α（TNF-α） in BMECs. The endothelial cells were treated with Caf at 25 μg/mL. The expression of ICAM-1 mRNA were detected by Real time RT-PCR,and ICAM-1 protein was measured by flow cytometry. Results： Car inhibited TNF-α-enhanced expression of ICAM-1 in BMECs. Conclusion ： Car decreases TNF-α-enhanced expression of ICAM- 1 in BMECs,which may be a protect effect on inflammatory injury after cerebral ischemia.