| 人脑神经干细胞的分离培养和鉴定 |
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| 引用本文： | 郑志竑,林玲,胡建石,陈贻锴,林建银.人脑神经干细胞的分离培养和鉴定[J].福建医科大学学报,2003,37(1):16-18,T001. |
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| 作者姓名： | 郑志竑 林玲 胡建石 陈贻锴 林建银 |
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| 作者单位： | 福建医科大学,分子医学研究中心,福州,350004 |
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| 基金项目： | 福建省科技重大项目 ( 2 0 0 1Z0 2 0 ) |
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| 摘 要： | 目的 探讨人脑神经干细胞的分离培养和鉴定方法。方法 采用无血清培养液从人胚胎脑皮质分离和培养神经干细胞，并应用免疫荧光技术进行细胞自我更新能力、巢蛋白表达和多向化潜能的鉴定。结果 鉴定表明从人胚胎脑皮质分离培养的细胞具有神经干细胞的特征，并可在体外增殖，经诱导可分化为神经元、星型胶质细胞和少突胶质细胞。结论 从人胚胎脑皮质成功分离培养了神经干细胞，它是研究细胞诱导分化的良好模型。
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| 关 键 词： | 脑 神经系统 干细胞 细胞培养 |
| 文章编号： | 1000-2235(2003)01-0016-03 |
Isolation, Culture and Characterization of Neural Stem Cells from Human Brain |
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| ZHENG Zhi\|hong,LIN Ling,HU Jian\|shi,CHEN Yi\|kai,LIN Jian\|yin.Isolation, Culture and Characterization of Neural Stem Cells from Human Brain[J].Journal of Fujian Medical University,2003,37(1):16-18,T001. |
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| Authors: | ZHENG Zhi\|hong LIN Ling HU Jian\|shi CHEN Yi\|kai LIN Jian\|yin |
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| Abstract: | Objective\ To isolate, culture and characterize the neural stem cells from human brain.\ Methods\ Neural stem cells from human embryonic cerebral cortex were isolated and cultured using serum\|free medium.\ Their self\|renewal ability, expression of nestin and multipotent of differentiation was identified with immunofluorescence staining.\ Results\ Neural stem cells were isolated from human embryonic cerebral cortex by serum\|free medium and capable of proliferate and long\|term survival in vitro.\ They could differentiate into neurons, astrocytes and oligodendrocytes.\ Conclusion\ Neural stem cells are successfully cultured from human embryonic cerebral cortex. |
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| Keywords: | brain neural system stem cell cell culture |
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