脑康胶囊对血管性痴呆大鼠行为学及海马区凋亡基因的影响

目的:探讨脑康胶囊对血管性痴呆大鼠行为学及海马区凋亡基因的影响。方法:健康SD大鼠,采用双侧颈总动脉永久性结扎方法制备血管性痴呆模型,随机分为假手术组、模型组、脑康组及银杏叶提取物组。ig 4周后,对各组大鼠进行Morris水迷宫行为学测定,用酶联免疫吸附试验(ELISA)检测大鼠海马组织超氧化物歧化酶(SOD),丙二醛(MDA)表达水平;用实时荧光定量核酸扩增(q PCR),免疫印迹法(Western blot)检测B淋巴细胞瘤-2(Bcl-2),Bcl-2相关X基因(Bax)mRNA,蛋白水平及Bcl-2/Bax。结果:与假手术组比较,模型组逃避潜伏期明显延长,穿越平台次数和平台象限路径百分比明显减少;SOD活性明显降低,MDA活性明显升高;Bax mRNA与蛋白水平显著增高,Bcl-2 mRNA与蛋白水平显著降低,Bcl-2/Bax降低。与模型组比较,脑康组逃避潜伏期明显缩短,穿越平台次数和平台象限路径百分比明显增加;SOD活性明显升高,MDA含量明显降低;Bax mRNA与蛋白水平显著降低,Bcl-2 mRNA与蛋白水平显著增高,Bcl-2/Bax升高。结论:脑康胶囊可显著改善VD大鼠的学习和记忆能力,其机制可能与抗氧化应激,抑制海马区细胞凋亡有关。

Effect of Naokang Capsule on Vascular Dementia and Hippocampal Apoptosis Genes in Rats

Objective: To observe the effect of Naokang capsule on vascular dementia and hippocampal apoptosis genes in rats. Method: Vascular dementia models were established by permanent bilateral common carotid arteries occlusion in healthy male sprague-dawley rats, then were randomly divided into sham-operation group, model group, Naokang capsule group and ginkgo biloba extract (EGB) group. Morris water maze (MWM) was used to evaluate the learning and memory ability of rats after 4 weeks of intragastric administration. Expression levels of superoxide dismutase (SOD) and malonaldehyde (MDA) in hippocampal tissues of rats were detected by enzyme linked immunosorbent assay (ELISA). The mRNA and protein expression of Bcl-2 and Bax as well as Bcl-2/Bax ratio in hippocampus were detected by Real-time quantitative PCR and Western blot. Result: Compared with sham-operation group, the escape latency of model group was significantly prolonged;frequency of crossing platform and swimming distance were obviously shortened, SOD activity was decreased significantly and MDA activity was increased significantly, mRNA and protein expression levels of Bax were significantly increased and the levels of Bcl-2 were significantly decreased, resulting in a decreased Bcl-2/Bax ratio in hippocampus. Compared with the model group, the escape latency of the rats in Naokang group was significantly shortened, frequency of the crossing platform and swimming distance were obviously increased, SOD activity was increased significantly and MDA activity was decreased significantly, mRNA and protein expression levels of Bax were significantly decreased and the levels of Bcl-2 were significantly increased, resulting in an increased Bcl-2/Bax ratio. Conclusion: Naokang capsule could significantly improve the learning and memory ability of vascular dementia rats and the mechanism may be related to antioxidant stress and inhibiting hippocampal apoptosis genes expression.