| Studies on the homogeneity between high-and lowaffinity glucocorticoid receptor |
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| 作者姓名： | 乐颖影 陆德如 徐仁宝 |
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| 作者单位： | Departments of Pathophysiology Second Military Medical University Shanghai 200433，Molecular Genetics，Second Military Medical University，Shanghai 200433，Departments of Pathophysiology Second Military Medical University，Shanghai 200433，Current address:Department of Physiology，First Military Medical University，Guangzhou 510515 |
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| 摘 要： | The homogeneity between the high-and low-affinity glucocorticoid receptors(GR_H and GR_L)was testified by immunoaffinity chromatography using Mab N250(recognizing the immunogenic domain at the N terminal of GR_H)and Mab BuGR1(recognizing the DNA binding domain of GR_H).The specific binding peak of 0.98μmol/L~3H]triamcinolone acetonide(TA)was higher than that of 52.50nmol/L~3H]TA.The re-sult suggests that the antigenic determinants of GR_L are similar to those of GR_H.ThecDNA of rat liver GR_H was introduced into GR_H-and GR_L-negative mouse fibroblast cellline E82.A3 by calcium phosphate coprecipitation.A number of clones which expressGR_H were selected with G418(400μg/ml).The results of radioligand binding assay indicatethat GR_H gene is expressed successfully and GR_L also may be encoded by GR_H gene.
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Studies on the homogeneity between high-and lowaffinity glucocorticoid receptor |
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| Le Yingying Lu Deru Xu Renbao.Studies on the homogeneity between high-and lowaffinity glucocorticoid receptor[J].Journal of Medical Colleges of PLA(China),1993(2). |
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| Authors: | Le Yingying Lu Deru Xu Renbao |
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| Institution: | Le Yingying Lu Deru Xu Renbao Departments of Pathophysiology~1 and Molecular Genetics~2,Second Military Medical University.Shanghai 200433 |
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| Abstract: | |
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| Keywords: | receptors glucocorticoid chromatography affinity receptor-deficient cell transfection |
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