人表皮黑素细胞培养条件的探讨

目的:比较培养基添加成分不同对体外培养黑素细胞生长的影响.方法:包皮取自小儿,采用分离酶和胰酶两步消化,加不同的添加成分的培养基,逐日观察黑素细胞增殖速度和树突伸展状态.结果:来自小儿包皮的黑素细胞易存活,增殖很快.胰酶和分离酶消化的标本,成纤维细胞较少,差胰酶处理去除角质形成细胞后为纯净的黑素细胞.采用添加人黑素细胞生长成分(HMGS)的254培养基,细胞增殖快,黑素细胞树突数目和分级增多.结论:小儿包皮用分离酶结合胰酶消化,以含HMGS的254培养基孵育可获得大量纯净的表皮黑素细胞.

Optimization of culture condition for human epidermis melanocytes growth in vitro

Objective:To investigate the effects of two mediums supplemented with various ingredient on epidermal melanocytes culture.Methods:The foreskins from an infant were treated with dispase II to separate epidermis from dermis,and treated with trypsin for obtaining single cell suspensions.The cells were cultured in different media supplemented with cell growth component.The proliferation and dendrites of cultured melanocytes were observed day by day.Results:The melanocytes from infant foreskin showed potent vitality.The fibroblasts cells in culture decreased after dispase and trypsin digesting specimen.The rapid proliferation and increased dendrites of melanocytes in 254 media supplemented with human melanocyte growth supplement(HMGS) were observed.Conclusions:Infant foreskins treated with dispase and trypsin and cultured with 254 containing HMGS,were suitable for melanocyte culture.