CIK/NK细胞4周培养过程中的CD27、CD28的表达及其意义

目的:了解细胞因子诱导杀伤（CIK）细胞和自然杀伤（NK）细胞在培养过程中CD27和CD28的表达变化规律。方法:通过流式细胞仪检测CD3+CD56+ CIK细胞以及CD3-CD56+ NK细胞在4周培养过程中其上CD27和CD28的表达变化。结果:CD27在CIK和NK细胞上均于培养的第2周达到最高峰，分别为（44.57±4.07）％和（51.02±5.20）％,但之后逐步下降，在培养第4周降至30%左右的水平。CD28在CIK和NK细胞上分别是在第3周和第2周时达到表达的最高峰，为（4.40±0.66）％和（45.14±3.58）％，之后迅速下降，在培养第4周已经几乎消失。结论:根据CD27和CD28在CIK/NK细胞上的表达变化规律，CIK/NK细胞的收获时机以培养第3周为宜。

Changes of the expression of CD27 and CD28 on cytokine-induced killer cells and natural killer cells during four week cultivation

AIM:To investigate the changes of CD27 and CD28 expression on cytokine-induced killer(CIK) cells and natural killer(NK) cells during cultivation.METHODS:The mononuclear cells were treated with interleukin-12(IL-2),IL-7,IL-15,stem cell factor and FLT3 ligand for four weeks.The CD27 and CD28 expression on CD3+CD56+ CIK cells and CD3-CD56+ NK cells were examined by flow cytometric analysis every week during four-week cultivation.RESULTS:The expression of CD27 on CIK and NK cells two weeks after cultivation reached the peak value at（44.57±4.07）% and（51.02±5.20）%, respectively.Then, their expression declined gradually to about 30% in the fourth week.The expression of CD28 on CIK and NK cells reached the peak at the third and the second week, the values were （4.40±0.66）% and （45.14±3.58）%, respectively, decreased rapidly and then were almost completely lost at the fourth week.CONCLUSION:According to the changes of expressions of CD27 and CD28 on CIK/NK cells, optimal harvest time of cultured CIK/NK cells should be at the third week of cultivation.