| 双向凝胶电泳技术在胰腺癌蛋白质组学研究中的应用及条件优化 |
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| 引用本文： | 宗美娟,孟猛. 双向凝胶电泳技术在胰腺癌蛋白质组学研究中的应用及条件优化[J]. 山东医药, 2010, 50(3): 20-22 |
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| 作者姓名： | 宗美娟 孟猛 |
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| 作者单位： | 1. 淄博职业学院,山东淄博,205013;山东省医药生物技术研究中心,卫生部生物技术药物重点实验室
2. 山东理工大学 |
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| 基金项目： | 山东省重大科技攻关课题资助项目 |
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| 摘 要： | 目的将双向凝胶电泳技术用于胰腺癌蛋白质组学研究并对其条件进行优化。方法对胰腺癌组织、癌旁组织、胰液等标本在组织研磨、裂解、蛋白抽提及双向凝胶电泳等各个环节进行调整和优化。结果液氮研磨法可得到更多的蛋白质点；超声方法去除组织中核酸效果较好；二硫苏糖醇（DTT）平衡时间12—15min，碘乙酰胺（IAA）平衡时间应等同或略长于DTT平衡时间时可得到高质量图谱；丙酮沉淀法可获得较多的胰液生物标志物蛋白点；将2—3根长度〈13cm的IPG胶条在1块凝胶上进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）的方法（Msoc）使双向电泳的重复性和通量显著提高。结论本研究成功建立了双向凝胶电泳技术平台，所获得的电泳图谱分辨率高、重复性好，能够较好地用于胰腺癌蛋白质组学研究。
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| 关 键 词： | 胰腺肿瘤 胰腺癌 双向凝胶电泳 蛋白质组学 |
Application and condition optimizing of two-dimensional gel electrophoresis in the study of pancreatic ductal adenocarcinoma proteomics |
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| ZONG Mei-juan,MENG Meng. Application and condition optimizing of two-dimensional gel electrophoresis in the study of pancreatic ductal adenocarcinoma proteomics[J]. Shandong Medical Journal, 2010, 50(3): 20-22 |
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| Authors: | ZONG Mei-juan MENG Meng |
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| Affiliation: | ZONG Mei-juan1,MENG Meng(1 Zibo Vocational Institute,Zibo 205013,P.R.China) |
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| Abstract: | Objective To put the two-dimensional(2D) gel electrophoresis(2-DE) into use for the study of pancreatic ductal adenocarcinoma(PDAC)proteomics,and to optimize the condition.Methods The samples of PDAC,normal tissue around PDAC and pancreatic juice were collected,and the sections including tissue grinding,lysising,proteins extraction and 2-DE were regulated and optimized.Results More proteins were obtained with grinding in liquid N,nucleic acid disturbation in tisssues were cleared up more effectively by ultr... |
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| Keywords: | pancreatic tumor pancreatic ductal adenocarcinoma two-dimensional gel electrophoresis proteomics |
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