磷酸化蛋白酪氨酸激酶在胆脂瘤上皮细胞中的表达

目的：从蛋白酪氨酸激酶(PTKs)信号传递系统的角度来探讨胆脂瘤上皮细胞增殖的分子机制。方法：采用免疫组织化学S-P方法和计算机图像分析系统，观察30例胆脂瘤上皮及19例胆脂瘤患者外耳道皮肤中磷酸化PTKs的表达。并结合炎症浸润、骨质破坏程度作统计学分析。结果：磷酸化PTKs呈棕黄色或棕褐色颗粒胞膜显色。与皮肤相比，胆脂瘤上皮细胞磷酸化PTKs的表达显著增强，且在不同的上皮及同一上皮的不同部位其表达具有不均一性，在重度炎症细胞浸润的上皮细胞磷酸化PTKs表达趋向于增强；不同的骨质破坏程度PTKs的表达差异无统计学意义。结论：胆脂瘤上皮细胞具有过度增殖能力。同时也存在抑制细胞增殖的机制；局部炎症可增强胆脂瘤上皮细胞增殖能力。

EXPRESSIONS OF PHOSPHATED PTKs IN CHOLESTEATOMA EPITHELIUM CELLS

Objective:To explore the cells proliferation and its molecular regulating mechanisms of cholesteatomatous epithelium from the aspect of protein tyrosine kinases (PTKs) signal transduction .Methods:The expressions of phosphated PTKs were investigated by immunohistochemical S P method and computer image analysis in 30 specimens of the cholesteatomatous epithelium and 19 specimens of external ear canal epithelium from the patients with cholesteatoma otitis media,The expressive results of phosphated PTKs were determined. Statistical analysis was performed by the connection with degree of subepidermal inflammatory cell infiltration and degree of bone destruction.Result:The expression of phosphated PTKs was primarily staining in cell membrane, The expressions of phosphated PTKs in cholesteatoma epithelium was clearly increased compared with that of external ear canal epithelium,and its expressions were uneven in different epitheliums as well as in different areas of same epithelium.The expression of phosphated PTKs tended to be strong in the epithelium with subepidermal inflammatory, the expressions of phosphated PTKs in cholesteatomatous epithelium were not significantly different under the different degree of bone destruction.Conclusion:The cells in cholesteatomatous epithelium have a unevenly hyperproliferation ability,and also show a mechanism of increased inhibiting proliferation ability. The inflammatory pathology are inferred to greatly affect proliferation ability of cholesteatomatous epidermis.