| 化学发光酶免疫测定法在妇科肿瘤中检测雌激素受体的研究 |
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| 引用本文： | 郑文,顾美皎,周宜开. 化学发光酶免疫测定法在妇科肿瘤中检测雌激素受体的研究[J]. 华中科技大学学报(医学版), 1996, 0(2) |
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| 作者姓名： | 郑文 顾美皎 周宜开 |
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| 作者单位： | 暨南大学医学院附属医院妇产科，同济医科大学附属同济医院妇产科，同济医科大学环境医学研究所 |
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| 摘 要： | 应用化学发光酶免疫分析法对妇科肿瘤组织雌激素受体进行定量测定，摸索了该法作为一种新的超微量检测技术的最佳反应条件，这些条件包括温度、时间及鲁米诺（ｌｕｍｉｎｏｌ）浓度。３４例标本经检测，结果在１０．９～３０６．５ｆｍｏｌ／ｍｇ之间。用本方法定量检测妇科肿瘤组织雌激素受体具有重要意义。
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| 关 键 词： | 妇科肿瘤；雌激素受体；化学发光酶免疫测定 |
A Study on Quantitative Determination of Estrogen Receptor of Gynecological Oncology with Chemiluminescent Enzyme Immunoassay |
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| Zheng Wen,Gu Meijiao, Zhou Yikai. A Study on Quantitative Determination of Estrogen Receptor of Gynecological Oncology with Chemiluminescent Enzyme Immunoassay[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 1996, 0(2) |
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| Authors: | Zheng Wen Gu Meijiao Zhou Yikai |
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| Abstract: | Chemiluminescent enzyme immunoassay(CLEIA)is a new supermicro quantitative technique which combines chemiluminescence with enzyme immunoassay. CLEIA is used for the quantitation of ER in gynecological oncological tissues. A lot of research has been done to determine the optimal conditions for reaction which consist of concentration of luminol, temperature and time;The results of ER in 34 specimens can be calculated through the value of individual luminosity by CLEIA and standard curve,the range of specimens is from 10.9 to 306.5 fmol/mg.CLEIA is of great significance in the quantitative determination of estrogen receptor in gynecological oncological tissues. |
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| Keywords: | gynecological oncology estrogen receptor chemiluminescent enzyme immunoassay |
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