Orthogonal test design for optimization of the extraction of flavonid from the Fructus Gardeniae

Objective It is imperative to provide some consistent experimental results for the extraction of flavonid from Fructus Gardeniae.Methods The key extraction parameters that influenced the yield of flavonid from Fructus Gardeniae were optimized by employing an orthogonal experiment [L9(3)4],including the ratio of buffer solution (Na2B4O7 · 10H2O) to raw material,concentration of Fructus Gardeniae in extracting solution,extraction time and pH of buffer solution.An UV/Vis detector was used to perform the qualitative and quantitative analyses of the extracted flavonid with the using of the standard sample.Results The maximum extraction yield of the crude extract was 5.0533 (mg/g) after 20 min when the mass ratio of Na2B4O7 · 10H2O to raw material was 0.4％,the concentration of Fructus Gardeniae in the extraction solution was 1/12 (g/mL),and pH of buffer solution was 4.5.The positive reactions to the Molish and HCI-Mg tests suggested that the extracted compound was flavonoid,and FTIR measurements also identified the presence of flavonoid in the extracts.Conclusion This work is expected to provide a basis for further research,development,and utilization of Fructus gardenia in flavonid extraction.