| Troglitazone Induced Apoptosis via PPARγ Activated POX-induced ROS Formation in HT29 Cells |
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| 作者姓名： | Wang J Lv X Shi J Hu X DU Y |
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| 基金项目： | supported by the National Basic Research Program (973) of China (No. 2009CB421605, No.2008CB418102); the National Natural Science Foundation of China (No.20890112, No. 21077127) |
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| 摘 要： | Objective In order to investigate the potential mechanisms in troglitazone-induced apoptosis in HT29 cells,the effects of PPARγ and POX-induced ROS were explored.Methods [3-(4,5)-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay,Annexin V and PI staining using FACS,plasmid transfection,ROS formation detected by DCFH staining,RNA interference,RT-PCR & RT-QPCR,and Western blotting analyses were employed to investigate the apoptotic effect of troglitazone and the potential role of PPARγ pathway...
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| 关 键 词： | 细胞凋亡 曲格列酮 PPAR 活性氧 诱导 激活 Western印迹 Annexin |
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