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Molecular characteristics and phylogenetic analysis of N gene of human derived rabies virus |
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| Authors: | Cai Liang Tao XiaoYan Liu YunZhi Zhang Hong Gao LiDong Hu ShiXiong Liu FuQiang Li Hao Shen XinXin Liu JiaHui Wang ShiQing Tang Qing |
| Affiliation: | CAI Liang 1,TAO XiaoYan 2,LIU YunZhi 1,ZHANG Hong 1,GAO LiDong 1,HU ShiXiong 1,LIU FuQiang 1,LI Hao 2,SHEN XinXin 2,LIU JiaHui 1,WANG ShiQing 1,and TANG Qing 2,1.Hunan Center for Disease Control and Prevention,Key Laboratory of Microbial Molecular Biology of Hunan Province,Changsha 410005,Hunan,China 2.State Key Laboratory for Molecular Virology and Genetic Engineering,Institute for Viral Disease Control and Prevention,China CDC,Beijing 102206,China |
| Abstract: | Objective To investigate the relationship between the molecular characteristics and phylogenetic evolution of rabies N gene.Methods Saliva samples were collected from rabies cases,and RT-PCR was used to amplify the N gene of rabies virus with the specific primers.The amplifying product of RT-PCR was cloned to pUCm-T vector and transformed into E.coli XL1-Blue and then the blue-white selection,PCR screening and gene sequencing were carried out to identify the positive clones.Finally,ExPASy and other bioinfor... |
| Keywords: | Rabies N gene Molecular Characteristics Phylogenetic |
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