结素蛋白多肽和微波技术在活动性结核病诊断中的意义

目的　探讨结素蛋白多肽抗原和微波辐射技术在诊断活动性结核的临床意义。方法 从结核菌标准株H37Rv培养物中提取结素蛋白多肽作抗原,进行微波辐射ELISA,检测活动性结核血清中结素蛋白多肽抗体水平。结果 126例活动性结核中114例呈阳性反应,76例健康人血清标本仅10例为阳性。其试验的灵敏性和特异性分别达到90.4%和86.8%。另外,微波ELISA耗时较常规法明显减少,但结果一致。结论 结素蛋白多肽抗原有较好的灵敏性和特异性,是ELISA的可靠抗原。微波ELISA可替代常规ELISA。

Applied value of tuberculin protein polypeptide and microwaves irradiation in detective of active tuberculosis

Objective To evaluate the diagnostic value of tuberculin protein polypeptide (TPP) and microwaves irradiation in detective of active tuberculosis.Methods Tuberculin protein polypeptide was drawed from culture medum of M.Tuberculosis H₃₇ Rv,was used microwaves-enzyme linked immunosorbent assay,to determine the titer of serum antibody against TPP in active tuberculosis.Results It was found that the tests were positive(≥1：80)in 114 of 126 active tuberculosis cases and in 10 of 76 healthy individuals.The sensitivity was 90.4% and the specificity was 86.8%.Besides,compared with the conventional-enzyme linked immunosorbent assay,the consumptive time of microwaves-enzyme linked immunosorbent assay was reduced considerably,but gain a consistent conclusion.Conclusion The antigen of TPP was proved to be better in sensitivity and specificity.Microwaves-enzyme linked immunosorbent assay can substitute conventional-enzyme linked immunosorbent assay.