去氢表雄酮通过调节丝/苏氨酸蛋白激酶信号传导通路抑制大鼠Morris肝细胞瘤生长

Objective To investigate the inhibitory effect of dehydroepaimdrosterone(DHEA)on the growth of transplatnted MorriS hepatomas(7288CTC)in vivo in rats.Methods 21 Buffalo rats were randomly devided into 4 groups,including one blank control group(n=5),one group for tumor-bearing control(n=6),and 2 experimental groups with DHEA(n=6)or DHEA-s(n=4).DHEA or DHEA-s was fed to the rats for 4 weeks immediately after Morris hepatomas(7288CTC)was implanted in both flanks.Phenotypes of the spleen lymphocytes were examined by flow cytometry,Akt and PTEN expression in rumor cells was detected by Western blot and immunohistochemistry.Results Tumor weights of DHEA treated group were less than those of the control(P＜0.05),the inhibitory rate was 43%.The results of Western blot and immunohistochemistry showed that in DHEA tumor group,the expression of phosphorilated Akt protein was decreased,the expression of PTEN was enhanced,the percentage of CD3 positive cells and the ratio of CD4/CD8 were increased (P＜0.05). Conclusion DHEA can inhibit tumor growth,possibly via the inhibition of the Akt signaling pathway as well as modulating the immune function.

The effect of DHEA on AKT signal pathway on transplanted Morris hepatomas in rats