胰岛素对人肝细胞L02水通道蛋白9表达的影响及其调控通路

Objective To explore the effects of insulin on the expression and the regulatory pathway of AQP9 in normal human liver cells. Methods Normal human liver cells L02 were cultured and treated with PI3K inhibitor LY294002, AKT inhibitor A-443654, MAPK inhibitors SB2030580 and insulin at different concentrations respectively. The AQP9 mRNA and protein expressions were detected with semi-quantitative RT-PCR andWestern blot respectively. Results The insulin (100nmol/L～500nmol/L) treatment decreased the expression of AQP9 in normal human liver cells (P < 0.05) concentration dependency, and the expression of AQP9 began to reduce from 3 hours of insulin stimulation (P < 0.05), especially at insulin treatment for 12 hours (P < 0.05); Incubated with the selective inhibitor of PI3K(LY294002) and AKT(A-443654), the inhibitory effects of insulin on AQP9 expression decreased (P < 0.05); but it did not change significantly by blocking the MAPK signaling pathway. Conclusion The insulin treatment inhibited the expression of AQP9 and the PI3K/akt signal transduction pathwsy was involved in the mechanism.

Effects of insulin and ly294002 inhibitors of PI3K on the regulations and expression of aquaporin 9 in normal liver cells