Physical maps and virulence of Anticarsia gemmatalis nucleopolyhedrovirus genomic variants |
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| Authors: | J E Maruniak A Garcia-Maruniak M L Souza P M A Zanotto F Moscardi |
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| Institution: | (1) Entomology and Nematology Department, University of Florida, Gainesville, Florida, U.S.A., US;(2) Empresa Brasileira de Pesquisa Agropequaria, EMBRAPA-CENARGEN, Sain, Brazil, BR;(3) EMBRAPA-CNPSo, Londrina, Brazil, BR |
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| Abstract: | Summary. Seventeen plaque purified isolates of two viral preparations of Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV), were analyzed in terms of the genomic changes after digestion of their DNAs with
HindIII and PstI restriction enzymes. The 1979 AgMNPV wild type preparation (AgMNPV-’79) resulted in six different variants
and the 1985 viral commercial preparation (AgMNPV-’85), in eleven. The genomic variation of all the isolates was mapped showing
that those from 1985 presented more heterogeneity with changes mapped in additional sites in comparison to the AgMNPV-’79
variants. Their virulence was compared by infecting two Lepidopteran cell lines, Spodoptera frugiperda (IPLB-SF-21AE) and Anticarsia gemmatalis (UFL-AG-286). The results indicated that there was some difference in virulence within the AgMNPV-’85 variants. This commercial
preparation had been applied in soybean fields in Brazil over several years to control the velvetbean caterpillar defoliation.
Accepted April 13, 1999 Received January 24, 1999 |
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